推荐产品
生物源
human
重組細胞
expressed in E. coli
化驗
≥95% (HPLC)
≥95% (SDS-PAGE)
形狀
lyophilized
效力
1x10^8 units/mg
分子量
~19.4 kDa
包裝
pkg of 100 μg
儲存條件
avoid repeated freeze/thaw cycles
雜質
endotoxin, tested
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
human ... IFNA2(3440)
一般說明
The gene IFN-α2a (interferon α 2a) is mapped to human chromosome 9. The encoded protein is a type 1 interferon and a glycoprotein. It belongs to the class of cytokines. Recombinant human IFN-α 2a is a single, non-glycosylated, polypeptide chain with a molecular mass 19.4kDa containing 166 amino acid residues. Interferon-α-2a, a recombinant interferon-a, is expressed in Escherichia coli. It is eliminated majorly via renal catabolism. However, hepatic and biliary elimination comprise the minor pathways.
應用
IFN-α2a (interferon α 2a)-human has been used to study heterosexual transmission of subtype C HIV (human immunodeficiency virus)-1.
IFN-alpha 2a human has been used:
- as standard protein in reverse-phase high-performance liquid chromatography (RP-HPLC) and reducing and non-reducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for characterizing recombinant consensus interferon (rh cIFN)
- as a supplement in Roswell Park Memorial Institute (RPMI) 1640 medium for studying interferon resistance in peripheral blood mononuclear cells (PBMC)
- to treat hepatocytes to test its effect on signal transducer and activator of transcription 1 (STAT1) mRNA levels and cytochrome P450 family 1 subfamily A member 2 (CYP1A2) expression
- to stimulate human monocyte derived macrophages (MDMs)
生化／生理作用
IFN-α2a (interferon α 2a) is a lymphoid factor that possesses potent anti-viral activity. It mainly interacts with cell surface-specific receptors. This interaction is responsible for anti-pathogenic response in cells. IFN-α2a also exhibits anti-tumor activities. Humans with brain tumor were shown to have mutations in this gene. Interferon-α-2a (IFN-a 2A) favors 2′5′-oligoadenylate synthetase and double-stranded RNA-dependent protein kinase induction to target viral mRNA and protein synthesis. IFN-α 2A exhibits immunomodulatory effects, inducing cytokines and other interferon production. It also increases natural killer and cytotoxic T cell activity, as well as histocompatibility complex antigen expression. It may be beneficial for treating hepatic cirrhosis, chronic hepatitis C and hepatitis D.
外觀
Lyophilized without any additives.
重構
Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute in water to a concentration of 0.1-1 mg/mL. This solution can then be diluted into other aqueous buffers.
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
C M Perry et al.
BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy, 10(1), 65-89 (2007-11-21)
Interferon-alpha-2a, a single interferon-alpha subtype manufactured by use of recombinant DNA technology, has immmunomodulatory, antiviral and antiproliferative properties. It is a beneficial treatment for about 30% of patients with well-compensated chronic hepatitis C. Biochemical responses [defined as normalisation of serum
Heterosexual Transmission of Subtype C HIV-1 Selects Consensus-Like Variants without Increased Replicative Capacity or Interferon-a Resistance.
Deymier MJ
PLoS Pathogens, 11, e1005154-e1005154 (2015)
Nadeem Ahmed et al.
Biotechnology and applied biochemistry, 62(5), 699-708 (2014-11-18)
Recombinant human consensus interferon (rh-cIFN) is an artificially engineered interferon (IFN) developed by recombining and reordering the protein sequences that exist in standard IFN. This recombination resulted into a drug that has the potential to work better than natural, standard
Maciej Czerwiński et al.
Pharmacology research & perspectives, 7(6), e00551-e00551 (2019-12-21)
Some immunomodulatory agents stimulate the release of cytokines capable of suppressing P450 enzymes and potentially affecting pharmacokinetics of coadministered medications. Cytokines released in response to an immunomodulator in the blood ex vivo can be used to screen for the potential
Mutations of the human interferon alpha-2b gene in brain tumor patients exposed to different environmental conditions.
Shahid S
Cancer Gene Therapy, 22, 246-261 (2015)
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