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生物源
human
重組細胞
expressed in E. coli
籤條
8-His tagged (C-terminus. )
化驗
≥80% (SDS-PAGE)
形狀
buffered aqueous solution
frozen liquid
分子量
~57.8 kDa
包裝
pkg of 10 μg
濃度
350 μg/mL
顏色
clear colorless
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
human ... MDM2(4193)
生化／生理作用
Originally discovered as one of three genes amplified on double minute chromosomes in a tumorigenic derivative of NIH 3T3 cells, MDM2 was later shown to possess oncogenic potential when overexpressed. High-level expression of MDM2 has also been shown to confer tumorigenic potential upon nontransformed rodent fibroblasts in athymic nude mice. MDM2 can immortalize rat embryo fibroblasts and can cooperate with activated RAS to transform these cells. Elevated levels of MDM2 protein have been found in a variety of human tumors, most notably in soft tissue sarcomas where up to 30% of primary tumors contain multiple copies of the MDM2 gene. One mechanism by which MDM2 overexpression promotes tumor development is through its ability to bind to the p53 tumor suppressor, thereby blocking the transactivation, cell cycle arrest, and apoptotic functions of p53. MDM2 can inhibit p53 activity in a number of ways including preventing p53 from recruiting TAFs, promoting nuclear export, inhibiting p53 acetylation, and perhaps most importantly by virtue of its function as an E3 ubiquitin ligase with specificity for, among others, p53. In addition to regulating p53 levels by targeting p53 for proteasomal degradation MDM2 also transfers ubiquitin to itself, MDMX, the ß2 adrenergic receptor, glucocorticoid receptor, TIP60, and PCAF.
外觀
Clear and colorless frozen liquid solution.
MDM2 is stored in 20mM Tris-Cl (pH 8.0), 20% Glycerol, 100mM KCl, 1mM DTT and 0.2mM EDTA buffer.
MDM2 is stored in 20mM Tris-Cl (pH 8.0), 20% Glycerol, 100mM KCl, 1mM DTT and 0.2mM EDTA buffer.
準備報告
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
C A Finlay
Molecular and cellular biology, 13(1), 301-306 (1993-01-01)
Expression of a p53-associated protein, Mdm-2 (murine double minute-2), can inhibit p53-mediated transactivation. In this study, overexpression of the Mdm-2 protein was found to result in the immortalization of primary rat embryo fibroblasts (REFs) and, in conjunction with an activated
L Cahilly-Snyder et al.
Somatic cell and molecular genetics, 13(3), 235-244 (1987-05-01)
We are exploring the origin and function of amplified DNA sequences associated with double minutes (DMs) in a spontaneously transformed derivative of mouse 3T3 cells. Toward that goal, we have constructed a cDNA library using RNA from these cells and
S S Fakharzadeh et al.
The EMBO journal, 10(6), 1565-1569 (1991-06-01)
We have carried out an analysis of amplified DNA sequences present in a tumorigenic mouse cell line, designated 3T3DM, to determine if the presence of cellular transforming activity is correlated with the elevated expression of any of the amplified genes.
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