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Merck
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主要文件

SRP0357

Sigma-Aldrich

NEK2 active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥65% (SDS-PAGE)

别名:

HsPK21, NEK2A, NIMA (never in mitosis gene a)-related kinase 2, NLK1

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.77

生物源

human

重組細胞

expressed in baculovirus infected Sf9 cells

化驗

≥65% (SDS-PAGE)

形狀

aqueous solution

分子量

53 kDa

包裝

pkg of 10 μg

儲存條件

avoid repeated freeze/thaw cycles

濃度

1.2 mg/mL

NCBI登錄號

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−70°C

基因資訊

human ... NEK2(4751)

一般說明

Human NEK2 (GenBank Accession No. NM_002497) full length with N-terminal HIS6-tag, MW=53 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

應用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

單位定義

One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of myelin basic protein (MBP) substrate per minute at pH 7.4 and 30°C.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Lynda Fletcher et al.
Biochimica et biophysica acta, 1744(2), 89-92 (2005-06-14)
Two splice variants of Nek2 kinase, a member of the NIMA-related family, have been identified as Nek2A and Nek2B. Nek2A regulates centrosome disjunction, spindle formation checkpoint signaling, and faithful chromosome segregation. A specific role for Nek2B has not yet been
Margarida Fardilha et al.
Annals of the New York Academy of Sciences, 1030, 468-478 (2005-01-22)
Mammalian sperm were previously shown to express the PP1gamma2 isoform of protein phosphatase 1 (PP1) as well as its regulatory proteins inhibitor 2 and glycogen synthase kinase 3. Furthermore, the development of sperm motility during transit through the epididymis correlates

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