SRP0169
JMJD2B Active human
recombinant, expressed in baculovirus infected insect cells, ≥40% (SDS-PAGE)
别名:
JHDM3B, JmjC domain-containing histone demethylation protein 3B, Jumonji domain-containing protein 2B, KIAA0876, Lysine-specific demethylase 4B (KDM4B)
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About This Item
分類程式碼代碼:
12352202
NACRES:
NA.32
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生物源
human
重組細胞
expressed in baculovirus infected insect cells
化驗
≥40% (SDS-PAGE)
形狀
aqueous solution
分子量
57 kDa
包裝
pkg of 100 μg
濃度
>0.02 mg/mL
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
human ... KDM4B(23030)
一般說明
Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No.NM_015015) (amino acids 1-500) with C-terminal FLAG tag, MW = 57 kDa, expressed in Sf9 cells using a Baculovirus expression system.
應用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
單位定義
One unit is defined as the amount of enzyme required to demethylate 1 pmol of substrate/min at 37°C.
外觀
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.
準備報告
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
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