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生物源
human
重組細胞
expressed in baculovirus infected insect cells
化驗
≥50% (SDS-PAGE)
形狀
aqueous solution
分子量
50 kDa
包裝
pkg of 20 μg
儲存條件
avoid repeated freeze/thaw cycles
濃度
>0.02 mg/mL
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
human ... SMYD3(64754)
一般說明
Human SMYD3 (GenBank Accession No. BC031010), full length, with N-terminal DDDDK tag (FLAG), MW = 50 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
應用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
外觀
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 100 μg/ml flag peptide, 20% glycerol and 3 mM DTT.
準備報告
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
International journal of molecular sciences, 21(24) (2020-12-19)
SMYD3 is a lysine methyltransferase that regulates the expression of over 80 genes and is required for the uncontrolled proliferation of most breast, colorectal, and hepatocellular carcinomas. The elimination of SMYD3 restores normal expression patterns of these genes and halts
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