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Merck
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Key Documents

SRP0133

Sigma-Aldrich

EZH2/EED inactive  human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

别名:

ENX-1, EZH2, Enhancer of zeste homolog 2, KMT6A, Lysine N-methyltransferase 6A

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.77

生物源

human

重組細胞

expressed in baculovirus infected insect cells

化驗

≥70% (SDS-PAGE)

形狀

aqueous solution

分子量

51 kDa (EED)
86 kDa (EZH2)

包裝

pkg of 20 μg

濃度

>0.02 mg/mL

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−70°C

基因資訊

human ... EZH2(2146)

一般說明

Complex of: Human EZH2 (GenBank Accession No. NM_004456), (amino acids 2-end) with N-terminal His tag, MW= 86 kDa. Human EED (GenBank Accession No. NM_003797) (amino acid 2-end) with N-terminal Flag tag, MW = 51 kDa, co-expressed in baculovirus expression system.

應用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

外觀

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 100 μg/ml flag peptide and 10% glycerol.

準備報告

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

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