SAB5201915

Monoclonal Anti-ERp57-Atto 390 antibody produced in mouse

clone 4F9, affinity isolated antibody, buffered aqueous solution

• 别名: Anti-PDIA3
• 分類程式碼代碼: 12352203
• NACRES: NA.45

属性

• 生物源: mouse
• 共軛: Atto 390 conjugate
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 4F9; monoclonal
• 描述: target molecular weight: 57 kDa
• 形狀: buffered aqueous solution
• 物種活性: human (Tested)
• 包裝: pkg of 100 μg
• 濃度: 1.0 mg/mL
• 技術: immunofluorescence: 1:100; western blot: 1:1000
• 同型: IgG2b
• Protein ID登錄號: NP_005304
• UniProt登錄號: P30101
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... PDIA3(2923)

说明

免疫原

Immunogen type: Recombinant protein
Immunogen Species: Human
Immunogen Region: See Notes section/Other
Immunogen Description: Human recombinant ERp57 (Grp58) protein corresponding to amino acids 25 - 505 (his-tagged)

應用

Western blotting
Dilution 1:1000
Tissue/Cell Type Used: HeLa cell lysate

Immunofluorescence
Dilution 1:100
Tissue/Cell Type Used: HeLa

標靶描述

ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface . ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus .

外觀

50% glycerol, 0.09% Sodium Azide

PBS pH 7.4

準備報告

Derived from cell/tissue culture

儲存和穩定性

Protect from light; avoid repeated freeze-thaw cycles. Store at -20 °C long-term, 2-8 °C short-term.

其他說明

Additional Product Details:
Immunogen region: Amino acids 25-505
Lot Expiration/Retest Date: Expires 1 year from purchase

Other:
Immunogen region: Amino acids 25-505

Purification method: Protein G purified
Product type: Primary antibody
Antibody structure: Whole Antibody
Protein ID: NP_005304.3

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2