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Merck

SAB4600116

Sigma-Aldrich

Monoclonal Anti-GFP IgG (H+L), CF 594 antibody produced in mouse

~2 mg/mL, clone 9F9.F9, purified immunoglobulin

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

mouse

共軛

CF 594 conjugate

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

9F9.F9, monoclonal

形狀

buffered aqueous solution

濃度

~2 mg/mL

技術

indirect immunofluorescence: 1-10 μg/mL

同型

IgG1κ

螢光

λex 593 nm; λem 614 nm

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

一般說明

Immunoglobulin G (IgG) belongs to immunoglobulin family and consists of gamma (γ) heavy chain in the constant (C) region. IgG is a widely expressed serum antibody. Monomeric structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa respectively, the primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, andγ4, respectively.1 IgG is produced and released from CD5+B cells, the predominant lymphocytes in the neonatal cell repertoire. Maternal IgG is the only antibody transported across the placenta to the fetus. Maternal IgG passively immunizes the infants.
CF 594 is a deep red fluorescent dye with molecular weight ~730. CF 594 is highly water soluble and photostable, therefore, it is considered to have a potential application in confocal microscopy and single molecule imaging. CF 594 is particularly useful in combination with blue fluorescent CF 350, green fluorescent CF 488A and far red CF 640R for multi-color imaging. CF 594 has excitation laser line at 532nm, 568nm or 594nm and absorption/emission maxima at 593/614nm.
Green fluorescent protein (GFP) is highly visible and efficiently emitting internal fluorophore. These key features of GFP have made it a potential marker of gene expression and protein targeting in intact cells and organisms.

特異性

Binds fluorescein

免疫原

recombinant GFP

特點和優勢

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

外觀

Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 2 mg/mL bovine serum albumin.

準備報告

Protect from light.

法律資訊

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
CF is a trademark of Biotium, Inc.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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其他客户在看

R Y Tsien
Annual review of biochemistry, 67, 509-544 (1998-10-06)
In just three years, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria has vaulted from obscurity to become one of the most widely studied and exploited proteins in biochemistry and cell biology. Its amazing ability to generate a
P Casali et al.
Current topics in microbiology and immunology, 210, 167-179 (1996-01-01)
Natural antibodies arise independently of known antigenic stimulation, are mostly IGM, polyreactive, and are generally encoded by V genes in germline configuration. Polyreactive IgM natural antibodies are produced by mainly B-1 cells which account for most of the B cell
Human placental Fc receptors and the transmission of antibodies from mother to fetus.
Simister NE and Story CM
Journal of Reproductive Immunology, 37(1), 1-23 (1997)
Antibody structure, instability, and formulation.
Wang W
Journal of Pharmaceutical Sciences, 96(1), 1-26 (2007)

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