SAB4200792

Anti-β-Tubulin IV antibody, Mouse monoclonal

clone ONS.1A6, purified from hybridoma cell culture

• 别名: Anti-TUBB4
• 分類程式碼代碼: 12352203
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 200
• 抗體表格: purified from hybridoma cell culture
• 抗體產品種類: primary antibodies
• 無性繁殖: ONS.1A6, monoclonal
• 形狀: buffered aqueous solution
• 分子量: ~55 kDa
• 物種活性: chicken, bovine, rat, mouse, human
• 技術: ELISA: suitable; immunoblotting: 10-20 μg/mL using human breast cancer MCF7 cell line. ; immunofluorescence: 5-10 μg/mL using human foreskin Hs68 cells.; immunohistochemistry: suitable
• UniProt登錄號: P04350
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... TUBB(203068) ; mouse ... Tubb3(22152)

说明

一般說明

Tubulin is the major building block of microtubules. It possesses an intracellular cylindrical filamentous structure that is present in almost all eukaryotic cells. Except in the simplest eukaryotes, Tubulin exists in all cells as a heterodimer of two similar but not identical polypeptides (approx. 55 kDa each), designated α and β, that assembles into microtubules. Both α and β Tubulins undergo post-translational modifications, including acetylation, phosphorylation, detyrosination, polyglutamylation and polyglycylation. For β-tubulin, six evolutionarily conserved isotypes were identified, designated βI-βVI. Their utilization in the same cell type of different species is nearly absolutely conserved with the exception of the sequenced diversed hematopoietic β-tubulin. Since the different isotypes of tubulin differ from each other in their ability to polymerize into microtubules, it is hypnotized that the β-tubulin isotypes contribute to unique functional properties. The most complex pattern of isotype distribution in tissues is seen in the vertebrate β-tubulins. In mammals βI is constitutive and found in most tissues. βII is found in many tissues, but largely in the brain; its synthesis increases in regeneration and development of neurons. βIII is found in the brain and in dorsal root ganglia; it appears to be localized to neurons, where its expression seems to increase during axonal outgrowth. βIV in mammals exists at two subtypes, differing from each other at 10 positions. βIVa is brain specific and βIVb is ubiquitous, but both appear to be constitutive. βV is ubiquitous expressed in a variety of cultured mammalian cells. βVI is apparently restricted to hematopoietic tissues, being expressed in mammalian platelets, spleen, bone marrow and other blood-forming tissues. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis, organellar and flagellar movement, intracellular transport and cytoskeletal functions. Antibodies reacting specifically with α- and β-tubulin isotypes serve as an essential tool in the detection of the presence and functional significance of these molecules in various cellular settings.

免疫原

Synthetic peptide corresponding to the C-terminal sequence of β-tubulin isotype IV, conjugated to BSA.

應用

Immunoblotting: a working concentration of 10-20 μg/mL is recommended using human breast cancer MCF7 cell line.
Immunofluorescence: a working concentration of 5-10 μg/mL is recommended using human foreskin Hs68 cells.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15mM sodium azide.

其他說明

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable