SAB4200415

Anti-NEZHA antibody, Mouse monoclonal

clone NEZHA-1, purified from hybridoma cell culture

• 别名: Anti-CAMSAP3, Anti-Calmodulin regulated spectrin-associated protein family member 3, Anti-KIAA, Monoclonal Anti-NEZHA antibody produced in mouse
• 分類程式碼代碼: 12352203
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified from hybridoma cell culture
• 抗體產品種類: primary antibodies
• 無性繁殖: NEZHA-1, monoclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~150 kDa
• 物種活性: canine, human
• 濃度: ~1.0 mg/mL
• 技術: immunoprecipitation (IP): suitable; indirect immunofluorescence: suitable; western blot: 2.5-5.0 μg/mL using whole extracts of human SW480 cells
• 同型: IgG1
• UniProt登錄號: Q9P1Y5
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... CAMSAP3(57662) ; mouse ... Camsap3(69697)

说明

一般說明

Monoclonal Anti-NEZHA (mouse IgG1 isotype) is derived from the hybridoma NEZHA-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the C-terminal region of human NEZHA, conjugated to KLH. Nezha (a deity in Chinese mythology) also known as KIAA1543 and calmodulin-regulated spectrin associated protein, is a 1276 amino acid protein that contains one calponin homology (CH) and two coiled coil (CC1 and 2) domains, as well as one DUF1781 (DUF) domain of unknown function. NEZHA is mapped to human chromosome 19p13.

NEZHA/ calmodulin regulated spectrin associated protein family member 3 (CAMSAP3) contains a conserved domain called cholecystokinin (CKK) domain at carboxy-terminal, as well as several coiled-coil regions and an calponin homology domain at amino-terminal.

免疫原

synthetic peptide corresponding to a sequence at the C-terminal region of human NEZHA, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey and dog NEZHA.

應用

Monoclonal Anti-NEZHA antibody produced in mouse has been used in immunofluorescence staining.

Monoclonal Anti-NEZHA antibody produced in mouse has been used in:

• immunofluorescence
• immunoprecipitation
• immunofluorescence
• mass-spectrometry-based analysis of a streptavidin pull-down assay
• immunostaining

生化／生理作用

CAMSAP3 (calmodulin-regulated spectrin-associated protein 3)/ NEZHA modulates the minus-end dynamics of microtubules. It helps to maintain neuronal polarity. Absence of this gene results in the formation of supernumerary axon.

NEZHA/ calmodulin regulated spectrin associated protein family member 3 (CAMSAP3) facilitates translocation of Golgi vesicles in epithelial cells. In addition, it also helps in positioning the apical-to-basal polarity of microtubules in epithelial cells.

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable