S5446

Anti-SUMO-1 (C-terminal) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• 别名: Anti-SMT3H3
• MDL號碼: MFCD02096449
• 分類程式碼代碼: 12352203
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: buffered aqueous solution
• 分子量: antigen 14 kDa
• 物種活性: human
• 技術: microarray: suitable; western blot: 1-2 μg/mL using nuclear extract of the human epitheloid carcinoma HeLa cell line
• UniProt登錄號: P63165
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: cow ... SUMO1(614967) ; human ... SUMO1(7341) ; mouse ... Sumo1(22218) ; rat ... Sumo1(301442)

说明

一般說明

Small ubiquitin-related modifier 1 (SUMO-1) is a highly conserved small ubiquitin-related modifier, also known as sentrin. SUMO-1 gene is mapped to human chromosome 2q33.1.

特異性

Anti-SUMO-1 (C-terminal) recognizes unconjugated SUMO-1 (14 kDa), SUMO-1-fusion protein, as well as proteins covalently conjugated to SUMO-1, eg. RanGAP1, 90kDa

免疫原

synthetic peptide corresponding to amino acids 86-97 located at the C-terminus of human SUMO-1, conjugated to KLH. This sequence is identical in many species including rat, mouse, bovine, chicken, and Xenopus, and highly conserved (single amino acid substitution) in C. elegans SMT3. The sequence has only 66% homology to human SUMO-2 and SUMO-3.

應用

Anti-SUMO-1 (C-terminal) antibody produced in rabbit has been used in immunoblotting.

生化／生理作用

Small ubiquitin-related modifier 1 (SUMO-1) is covalently conjugated to the lysine side in proteins through its C-terminal glycine residue by an isopeptide bond. Their conjugation with cellular proteins has been correlated wide variety of biological processes including inflammation, oncogenesis nuclear transport, cell cycle control and viral infection response. SUMO-1 serves as a substrate for Ran GTPase-activating protein (RanGAP1). Unmodified RanGAP1 is present in the cytoplasm, suggesting that modification by SUMO-1 may target RanGAP1 to the nuclear pore complex (NPC). Haploinsufficiency in the SUMO-1 gene is implicated in the Cleft Lip and Palate.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

儲存和穩定性

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.