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物種活性
human
包裝
kit of 96 wells (12 strips x 8 wells)
技術
ELISA: suitable
capture ELISA: suitable
輸入
sample type serum
sample type plasma
sample type urine
sample type cell culture supernatant(s)
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 50 pg/mL
standard curve range: 74.07-18000 pg/mL
檢測方法
colorimetric
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
human ... SPP1(6696)
一般說明
The Human OPN ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human OPN in serum, plasma, cell culture supernatants and urine.
免疫原
Recombinant Human OPN
應用
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human Osteopontin ELISA Kit has been used in the quantification of serum osteopontin (OPN) in human blood samples.
生化／生理作用
Osteopontin (OPN) is a secreted glycoprotein and is assumed to be associated with various cellular processes such as adhesion, migration, and biomineralization in diverse tissues. Osteopontin acts as a ligand to αvβ3 integrin and possibly other receptors. In addition to its involvement in mineralized matrix formation, osteopontin can also function as a cytokine that stimulates the release of IFNγ (interferon γ) and IL-12 (interleukin 12), while inhibiting the production of IL-10. Osteopontin is involved in inflammation and also regulates immunity against infectious diseases. It also co-stimulates T cell proliferation. Osteopontin has proinflammatory functions and is associated with multiple sclerosis. It participates in inflammation in Crohn′s Disease. Osteopontin plays a crucial role in bone remodelling and retards ectopic calcification by inhibiting the formation of hydroxyapatite. OPN is thought to prevent renal stone formation by suppressing nucleation, growth and aggregation of calcium oxalate crystals.
其他說明
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
訊號詞
Warning
危險聲明
防範說明
危險分類
Met. Corr. 1
儲存類別代碼
8A - Combustible corrosive hazardous materials
Can Osteopontin Be Considered a Biomarker for Endometriosis?
Albu D R, et al.
Revista de Chimie (Bucharest, Romania), 13(3), 1-1 (2017)
Octavio Gamaliel Aztatzi-Aguilar et al.
PloS one, 16(3), e0249057-e0249057 (2021-03-26)
Osteopontin (OPN) is a protein involved in inflammatory illnesses such as fibrosis and cancer; its overexpression in cardiovascular diseases promotes the biomineralization of blood vessels and other soft tissues. Moreover, there is an active component of oxidative stress related with
Post-translational modification and proteolytic processing of urinary osteopontin.
Christensen B, et al.
The Biochemical Journal, 411, 53-61 (2008)
The adhesive and migratory effects of osteopontin are mediated via distinct cell surface integrins. Role of alpha v beta 3 in smooth muscle cell migration to osteopontin in vitro.
Liaw L, et al.
The Journal of Clinical Investigation, 95, 713-724 (1995)
The influence of the proinflammatory cytokine, osteopontin, on autoimmune demyelinating disease.
Chabas D, et al.
Science, 294, 1731-1735 (2001)
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