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Merck
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主要文件

P8563

Sigma-Aldrich

Polyuridylic acid–Agarose

lyophilized powder, matrix polyacrylhydrazido-agarose

别名:

Poly(U)–Agarose potassium salt

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About This Item

MDL號碼:
分類程式碼代碼:
41106500
NACRES:
NA.56

形狀

lyophilized powder

品質等級

標籤範圍

0.1-1.0 mg per mL

基質

polyacrylhydrazido-agarose

基質結合

ribose moiety after periodate oxidation

膨潤

1 g swells to ~4 mL

容量

≥0.2 mg/mL binding capacity (poly(A) (P 9403))

儲存溫度

−20°C

應用

Polyuridylic acid-agarose is used in protein chromatography, affinity chromatography and specialty resins. Polyuridylic acid has been used to study insulin receptors and to develop polyclonal antibodies against RNase L.

外觀

用乳糖稳定化的冻干粉

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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L P Gavrilova et al.
FEBS letters, 178(2), 283-287 (1984-12-10)
The stoichiometry of GTP hydrolysis during peptide elongation in the processes of codon-specific translation and misreading of polyuridylic acid was determined in a cell-free system in which all ribosomes were active in peptide synthesis. Ribosomes carrying oligophenylalanine presynthesized on poly(U)
N J Horscroft et al.
The Journal of general virology, 81(Pt 8), 1961-1965 (2000-07-19)
The bluetongue virus ssRNA-binding protein, NS2, is a phosphoprotein that forms viral inclusion bodies in infected cells. Recombinant NS2 was expressed in the baculovirus expression system and purified to homogeneity from insect cells. Purified NS2 bound nucleosides. Further investigation revealed
Eulàlia Belloc et al.
Nature, 452(7190), 1017-1021 (2008-04-04)
In vertebrate oocytes, meiotic progression is driven by the sequential translational activation of maternal messenger RNAs stored in the cytoplasm. This activation is mainly induced by the cytoplasmic elongation of their poly(A) tails, which is mediated by the cytoplasmic polyadenylation
J P Medema et al.
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, 7(4), 543-550 (1996-04-01)
We have used two approaches to identify possible substrates of the insulin receptor (IR) tyrosine kinase. First, we used a potent tyrosine phosphatase inhibitor, phenylarsine oxide (PAO), which is reported to be specific for the insulin-induced signal transduction route, to
T Salehzada et al.
The Journal of biological chemistry, 266(9), 5808-5813 (1991-03-25)
RNase L activated by 2-5A (a series of 2'-5'-linked adenylic oligoribonucleotides) is a key enzyme of the interferon system. To study RNase L (endonuclease L) in intact cells independently of intracellular 2-5A and of its activity, we have developed polyclonal

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