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P5400

Sigma-Aldrich

果胶酯酶 来源于橘皮

greener alternative

lyophilized powder, ≥150 units/mg protein

别名:

Pectin methylesterase, 果胶甲酯酶

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About This Item

CAS号:
9025-98-3
EC 号:
3.1.1.11 (BRENDA, IUBMB)
EC號碼:
232-807-0
MDL號碼:
MFCD00131812
分類程式碼代碼:
12352204
NACRES:
NA.54

形狀

lyophilized powder

品質等級

200

比活性

≥150 units/mg protein

成份

Protein, 20-50% biuret

環保替代產品特色

Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

環保替代類別

, Enabling

儲存溫度

2-8°C

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一般說明

我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。该产品已用作替代能源的酶。更多信息，请参阅 替代能源的酶研究

應用

在测定沙棘果汁中果胶甲酯酶活性时，使用 Sigma 酶作为标准。它被用于细胞壁果胶的水解，同时研究 拟南芥中的叶状结构 。它也被用于催化果胶水解（使用超声波测量装置），以监测生物过程。
果胶酯酶用于将果胶的甲酯水解为果胶酯和甲醇。含有果胶甲酯酶和果胶果胶水解酶。产品 P5400 来自橘皮，为冻干粉。包含 (NH4)2SO4 和氯化钠。已用于果胶甲基酯酶基因的研究 。

生化／生理作用

果胶酯酶催化果胶的甲酯水解产生果胶和甲醇。

單位定義

在 pH 7.5、30°C 条件下，1 个单位每分钟从果胶中释放 1.0 μg 酸。

外觀

含 (NH4)2SO4 和氯化钠

分析報告

由缩二脲测定的蛋白质。

其他說明

查看更多关于 复合糖分析用酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析证书(COA)

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醇氧化酶 溶液 来源于巴斯德毕赤酵母 buffered aqueous solution, 10-40 units/mg protein (biuret)

Sigma-Aldrich

A2404

醇氧化酶 溶液 来源于巴斯德毕赤酵母

Kerstin Müller et al.
Plant physiology, 161(1), 305-316 (2012-11-07)
The methylesterification status of cell wall homogalacturonans, mediated through the action of pectin methylesterases (PMEs), influences the biophysical properties of plant cell walls such as elasticity and porosity, important parameters for cell elongation and water uptake. The completion of seed
Sergio T de Freitas et al.
The Plant journal : for cell and molecular biology, 71(5), 824-835 (2012-05-09)
Blossom-end rot (BER) in tomato fruit (Solanum lycopersicum) is believed to be a calcium (Ca²⁺) deficiency disorder, but the mechanisms involved in its development are poorly understood. Our hypothesis is that high expression of pectin methylesterases (PMEs) increases Ca²⁺ bound
Yuan Zhou et al.
Phytochemistry, 76, 46-51 (2012-01-11)
Aluminum (Al) toxicity is one of the most widespread problems for crop production on acid soils, and nitric oxide (NO) is a key signaling molecule involved in the mediation of various biotic and abiotic stresses in plants. Here we found
Yuri L Dorokhov et al.
PLoS pathogens, 8(4), e1002640-e1002640 (2012-04-13)
Many plants release airborne volatile compounds in response to wounding due to pathogenic assault. These compounds serve as plant defenses and are involved in plant signaling. Here, we study the effects of pectin methylesterase (PME)-generated methanol release from wounded plants
Doungla E Ngouémazong et al.
Carbohydrate research, 348, 69-76 (2012-01-03)
The influence of the degree and pattern of methylesterification (DM and PM, respectively) on the stiffness of Ca(2+)-pectin gels is extensively examined, at various Ca(2+) concentrations. Accordingly, a highly methyl-esterified pectin was selectively de-esterified using NaOH, plant or fungal pectin
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Carbohydrate Analysis

A key resource feature at our Enzyme Explorer section of biochemicals is "Enzymes for Carbohydrate Analysis and Digestion." Offering kits, reagents, analysis, lists of enzymes related to PTM and carbohydrate metabolism.

Carbohydrate Analysis

A key resource feature at our Enzyme Explorer section of biochemicals is "Enzymes for Carbohydrate Analysis and Digestion." Offering kits, reagents, analysis, lists of enzymes related to PTM and carbohydrate metabolism.

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