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應用
Immobilon-P 聚偏二氟乙烯膜已用于蛋白质印迹,用在进行了十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)后转移蛋白质的过程中。
聚偏二氟乙烯膜是蛋白免疫印迹、 和测序 的常用膜。蛋白质与 PVDF 结合的基础被认为是由于疏水相互作用。用于免疫印迹的 PVDF 膜有最常用的 0.45μm 孔径。PVDF 膜具有更大的蛋白结合能力,比硝酸纤维素膜更耐用,更耐溶剂。
法律資訊
Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
Cross-phosphorylation between Arabidopsis thaliana sucrose nonfermenting 1-related protein kinase 1 (AtSnRK1) and its activating kinase (AtSnAK) determines their catalytic activities.
The Journal of Biological Chemistry, 285, 12071-12077 (2010)
Infection and immunity, 77(6), 2488-2498 (2009-04-01)
Parasitic worms and molecules derived from them have powerful anti-inflammatory properties and are shown to have therapeutic effects on inflammatory diseases. The helminth Fasciola hepatica has been reported to suppress antigen-specific Th1 responses in concurrent bacterial infections, thus demonstrating its
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