推荐产品
等級
for molecular biology
品質等級
化驗
≥99.0%
形狀
liquid
pH值
6.5-6.9(pH of phenolic phase)
7.8-8.2(pH of phenolic phase after the addition of Equilibration Buffer)
應用
agriculture
儲存溫度
2-8°C
應用
对于需要更高 pH 值的应用，例如大的完整基因组 DNA的分离，建议添加平衡缓冲液。
苯酚：氯仿：异戊醇已用于制备 RNA，用于研究从心肌活检中分离总 RNA。它还被用于在人雄激素受体（HUMARA） 克隆性检测中提取 DNA。
包裝
随配 P3803 和一瓶单独的平衡缓冲液，用于将苯酚相调节至 pH 8.0 ± 0.2。
適合性
这批苯酚：氯仿：异戊醇 25:24:1 采用从大肠杆菌中提取 的pBR322 DNA 的粗制品进行了测试。 根据琼脂糖凝胶电泳评估，提取的 DNA 没有降解或浓度降低。 采用相同的操作程序处理纯化的 pBR322 DNA 和 λ Hind III 消化 DNA。 根据琼脂糖凝胶电泳评估，纯化的制剂没有检测到降解或浓度降低。
準備報告
将小瓶平衡缓冲液的全部内容物添加到大瓶苯酚：氯仿：异戊醇中。 在使用前轻柔地混合并使相实现分离，大约 2-4 小时。 这会将苯酚溶液的 pH 值从 6.7± 0.2 调整到 8.0± 0.2。
其他說明
添加平衡缓冲液后，产品可稳定 6 个月，而不添加缓冲液可稳定 2 年。 在添加平衡缓冲液后，在 -20 °C 下避光储存可将保质期延长至 1 年以上。
相關產品
产品编号
说明
价格
訊號詞
Danger
危險分類
Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3
標靶器官
Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin
儲存類別代碼
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
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The potency and specificity of locked nucleic acid (LNA) antisense oligonucleotides was investigated as a function of length and affinity. The oligonucleotides were designed to target apolipoprotein B (apoB) and were investigated both in vitro and in vivo. The high
实验方案
Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.
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