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生物源
Porcine gastric mucosa
品質等級
形狀
lyophilized powder
比活性
≥30 units/mg dry solid
分子量
35 kDa
基質
4% cross-linked beaded agarose
UniProt登錄號
儲存溫度
−20°C
基因資訊
pig ... LOC396892(396892)
相关类别
一般說明
胃蛋白酶是一种在胃液分泌物中发现的酶。
應用
固定在琼脂糖珠上的胃蛋白酶可用于消化并随后从糖蛋白麦醇溶蛋白中分离出活性肽。
用于产生抗体的F(ab′)2 片段。1
胃蛋白酶-琼脂糖已作为固定工具用于研究胃蛋白酶的重折叠能力。它还用于从兔IgG制备F(ab′)2片段。
生化／生理作用
优先将C末端切割成Phe、Leu和Glu。它不会在Val、Ala或Gly上进行切割。最佳pH2-4。活性成分为4 M尿素和3 M盐酸胍。在60°C时稳定。胃蛋白酶在pH > 6时会不可逆地失活。
單位定義
在pH2.0，37℃条件下用血红蛋白作为底物，对TCA可溶性产物进行测定时，一个单位每分钟可产生0.001 ΔA的280（终体积 = 16 mL。光程 = 1 cm。）
數量
一克固体可膨胀至5-10ml填充凝胶。
外觀
用乳糖稳定化的冻干粉
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
其他客户在看
M Feldman et al.
Gastroenterology, 110(4), 1043-1052 (1996-04-01)
Recent studies suggesting that gastric secretion does not decrease with aging included few elderly individuals and measured only acid secretion. The aims of this study were to measure gastric acid and pepsin output in 206 health Americans (age range, 18-98
Ludmila Tucková et al.
Journal of leukocyte biology, 71(4), 625-631 (2002-04-03)
Celiac disease, induced by dietary gluten, is characterized by mucosal atrophy and local inflammation associated with cell infiltration and activation. Unlike other food proteins, gluten and its proteolytic fragments, besides inducing a specific immune response, were shown to activate components
Thomas A Kraus et al.
The Journal of clinical investigation, 115(8), 2234-2243 (2005-07-26)
To explore the requirement for M cells and the Peyer's patch (PP) in induction of oral tolerance and address the potential in vivo role of intestinal epithelial cells as nonprofessional APCs, we have attempted to induce tolerance in mice with
Hana Dvořáková et al.
Frontiers in cellular and infection microbiology, 10, 66-66 (2020-03-17)
Schistosomula (the post-infective stages) of the neurotropic schistosome Trichobilharzia regenti possess multiple isoforms of cathepsin B1 peptidase (TrCB1.1-TrCB1.6) with involvement in nutrient digestion. The comparison of substrate preferences of TrCB1.1 and TrCB1.4 showed that TrCB1.4 had a very narrow substrate
Gastric proteinases--structure, function, evolution and mechanism of action.
B Foltmann
Essays in biochemistry, 17, 52-84 (1981-01-01)
实验方案
This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.
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