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Merck

P0091

Sigma-Aldrich

PolyFreeze

water-soluble support matrix

别名:

water-soluble support matrix for frozen sectioning

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About This Item

分類程式碼代碼:
12171500
NACRES:
NA.47

品質等級

顏色

colorless

密度

1.030 g/cm3

應用

hematology
histology

儲存溫度

room temp

一般說明

PolyFreeze is a water-soluble support matrix and a form of embedding medium for frozen sectioning. PolyFreeze sections flawlessly at routine frozen specimen thickness of 5-6 μ and leaves no slide residue. It provides for -20 ºC cryostat sectioning in rapid set embedment. This medium freezes quickly supporting the tissue for sectioning at 3 mm and up with no cracking of the matrix at temperatures from -8 °C to -25 °C. Snap freeze using PolyFreeze with isopentane and liquid nitrogen, or dry ice (slush/slurry or bunker). Store specimens frozen in PolyFreeze in liquid nitrogen canisters or in airtight containers in a -80 °C freezer. PolyFreeze does not cause autofluorescence and can easily be washed away during fixation and rinsed prior to staining. Once rinsed, there is no trace of the support matrix to interfere with staining or immunohistochemistry (IHC) reactions.

應用

PolyFreeze has been used as an embedding medium for frozen sectioning of plant and animal samples.

準備報告

PolyFreeze is supplied ready-to-use. A cloudy appearance of the unfrozen product has no effect on its performance.

儲存和穩定性

Store the product at room temperature

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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