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MIDI67

Sigma-Aldrich

PKH67 Green Fluorescent Cell Linker Midi Kit，用于常规细胞膜标记

Distributed for Phanos Technologies

别名:

Green PKH membrane midi labeling kit

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.32

包裝

pkg of 1 kit

品質等級

100

製造商／商標名

Distributed for Phanos Technologies

儲存條件

protect from light

技術

flow cytometry: suitable

螢光

λex 490 nm; λem 502 nm (PKH67 dye)

應用

cell analysis
detection

檢測方法

fluorometric

運輸包裝

ambient

儲存溫度

room temp

相关类别

一般說明

此试剂盒用于常规细胞膜标记。PKH67具有比PKH1和PKH2（之前在体外和体内细胞示踪应用体积的另外两种绿色染料）更长的脂肪族碳尾。由于具有更长的尾部，内部研究一致表明与PKH2相比，PKH67的细胞转移减少。
使用PKH1和PKH2进行的体内研究观察到荧光缓慢消失。PKH67可能表现出相似性质，因为这种行为似乎是绿色细胞接头染料的特征，而非红色细胞接头染料的特征。 非分裂期细胞的体外细胞膜的保留性质与体内荧光半衰期之间的相关性，预测PKH67的体内荧光半衰期为10-12天。 其他半衰期相似的绿色细胞接头染料已用于监测1-2个月内体内淋巴细胞和巨噬细胞运输，表明PKH67还可用于中等长度的体内示踪研究。

應用

PKH67绿色荧光细胞接头试剂盒(Midi)已用于：
  • 标记外泌小体  
  • 胞外囊泡(EV)l
  • 正常和镰状红细胞 (RBCs)
  • Raji cells细胞

聯結

如需关于PKH及CellVue®的荧光细胞连接子染料的更多技术详情，包括大量的参考书目，请访问这里。

法律資訊

CellVue is a registered trademark of Phanos Technologies

仅试剂盒组分

产品编号
说明
  • PKH67 Dye 2 x 0.1
  • Diluent C 6 x 10

象形圖

FlameExclamation mark
GHS02,GHS07

訊號詞

Danger

危險聲明

H225,H319

危險分類

Eye Irrit. 2 - Flam. Liq. 2

儲存類別代碼

3 - Flammable liquids

閃點（°F）

57.2 °F - closed cup

閃點（°C）

14 °C - closed cup

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Dongxin Tang et al.
Clinical and translational medicine, 10(4), e139-e139 (2020-09-09)
Cancer stem cells (CSCs) are important factors contributing to tumorigenesis. We examined whether CSCs isolated from colorectal cancer (CRC) cells possess metastatic properties that can be transferred to non-CSCs via the delivery of miR-200c enclosed in extracellular vesicles (EVs). The
Notochordal-cell derived extracellular vesicles exert regenerative effects on canine and human nucleus pulposus cells
Bach F, et al.
Testing, 8(51), 88845-88845 (2017)
Modulation of Sickle Red Blood Cell Adhesion and its Associated Changes in Biomarkers by Sulfated Nonanticoagulant Heparin Derivative
Alshaiban A, et al.
Clinical and Applied Thrombosis/Hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis, 22, 230-238 (2016)
ADCC Assay Protocol
Fang L and He F
Journal of Virology (2013)
Chuang Gao et al.
Aging, 11(24), 12147-12164 (2019-12-17)
Exosomes are small (30-150 nm diameter) lipid bilayer-enclosed vesicles found in all bodily fluids. We investigated whether exosomes play a role in chronic subdural hematoma (CSDH). Exosomes were identified and characterized using transmission electron microscopy and NanoSight particle tracking. The
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