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一般說明
Imprint™ 甲基化 DNA 定量技术提供了一种快速可靠的方法来测量全局 DNA 甲基化移位。印记甲基化 DNA 定量试剂盒包含检测相对水平的甲基化 DNA 所需的所有试剂。最多 200 ng 纯化 DNA 与检测试剂条的微孔结合。使用捕获和检测抗体检测甲基化 DNA，然后用比色法定量。样本中存在的甲基化 DNA 的量与测量的吸光度成正比。
應用
Imprint® 甲基化 DNA 定量试剂盒已用于全局 DNA 甲基化分析。
特點和優勢
- 基于 ELISA 的格式
- 在 4 个易于遵循的步骤中完成程序
- 提供的所有试剂，包括对照甲基化 DNA
- 检测限为 5 ng 全甲基化 DNA
- 输入 DNA 可能低至 10-200 ng
- 程序在 3.5 小时内完成
- 96 孔格式允许研究单样本或高通量研究
- 对 5-mC 具有高特异性的优化抗体和试剂；对非甲基化胞嘧啶无交叉反应
- 可与贴壁和悬浮细胞、组织、血浆及其他体液如唾液、尿液和血清一起使用
法律資訊
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
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危險分類
Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1
儲存類別代碼
8A - Combustible corrosive hazardous materials
Kazuhisa Nakano et al.
Journal of immunology (Baltimore, Md. : 1950), 190(3), 1297-1303 (2013-01-02)
Rheumatoid arthritis (RA) is a chronic inflammatory disease in which fibroblast-like synoviocytes (FLS) exhibit an aggressive phenotype. Although the mechanisms responsible are not well defined, epigenetic determinants such as DNA methylation might contribute. DNA methyltransferases (DNMTs) are critical enzymes that
Vitalina M Komashko et al.
Epigenetics, 5(3), 229-240 (2010-03-23)
Methylation of DNA in combination with histone modifications establishes an epigenetic code that ensures the proper control of gene expression. Although DNA methyltransferases have been shown to interact with histone methyltransferases such as EZH2 (which methylates histone H3 on lysine
INSIG2 rs7566605 single nucleotide variant and global DNA methylation index levels are associated with weight loss in a personalized weight reduction program
Pirini F, et al.
Molecular Medicine Reports, 17(1), 1699-1709 (2018)
Rafael Guerrero-Preston et al.
Epigenetics, 5(6), 539-546 (2010-06-05)
Environmental exposures in-utero may alter the epigenome, thus impacting chromosomal stability and gene expression. We hypothesized that in utero exposures to maternal smoking and perfluoroalkyl compounds (PFCs) are associated with global DNA hypomethylation in umbilical cord serum. Our objective was
Magdalena Gryzinska et al.
Biochemical genetics, 51(7-8), 554-563 (2013-04-05)
DNA methylation is an epigenetic modification that plays an important role in the normal development and function of organisms. The level of DNA methylation is species-, tissue-, and organelle-specific, and the methylation pattern is determined during embryogenesis. DNA methylation has
商品
The overall degree of methylation of a genome can be a useful measure of global regulatory changes. Measurement of this parameter is usually performed after complete digestion to the single base and then analyzed using HPLC or mass spectrophotometry. The Global DNA Modification Kit (MDQ1), allows the researcher to monitor DNA methylation using a format similar to a sandwich ELISA assay.
实验方案
Imprint ® Methylated DNA Quantification Kit (MDQ1) Protocol
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
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