MAK538

Ammonia Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• 分類程式碼代碼: 12161503
• NACRES: NA.84

属性

• 品質等級: 100
• 檢測方法: colorimetric; fluorometric
• 相關疾病: gastrointestinal diseases
• 運輸包裝: wet ice
• 儲存溫度: −20°C

说明

一般說明

Ammonia (NH3) or its ion form ammonium (NH4 +) is an important source of nitrogen for living systems. It is synthesized through amino acid metabolism and is toxic when present at high concentrations. In the liver, ammonia is converted to urea through the urea cycle. Elevated levels of ammonia in the blood (hyperammonemia) have been found in liver dysfunction (cirrhosis), while hypoammonemia has been associated with defects in the urea cycle enzymes (e.g. ornithine transcarbamylase).

特點和優勢

• Linear detection range: 24 to 1000 µM

適合性

The kit is suitable for ammonia determination in biological samples (e.g. serum, plasma, urine, saliva, cell culture, etc).

原則

The Ammonia Assay Kit is designed to directly measure NH₃ and NH4 +. In this assay, NADH is converted to NAD⁺ in the presence of NH₃, ketoglutarate and glutamate dehydrogenase. The decrease in optical density at 340 nm or fluorescence intensity at λEx = 360 nm/λEm = 450 nm is directly proportionate to the NH3 concentration in the sample.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3