推荐产品
生物源
mouse
品質等級
共軛
unconjugated
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
9B10, monoclonal
形狀
buffered aqueous solution
分子量
antigen ~85 kDa
物種活性
human
濃度
~2 mg/mL
技術
immunocytochemistry: 10-20 μg/mL using HeLa cells
immunoprecipitation (IP): suitable
microarray: suitable
同型
IgG2b
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... MAD1L1(8379)
一般說明
Mitotic arrest deficient-like 1 (MAD1) is part of the cell-cycle checkpoint.
Monoclonal Anti-Mad1 (mouse IgG2b isotype) is derived from the hybridoma 9B10 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with human recombinant Mad1 protein.
免疫原
recombinant human Mad1 protein.
應用
Monoclonal Anti-Mad1 antibody produced in mouse has been used in
- immunoblotting
- immunoprecipitation
- immunocytochemistry
生化／生理作用
Mitotic arrest deficient-like 1 (MAD1) functions as a regulator in the segregation of chromosomes during mitosis. It recruits mitotic arrest deficient-like 2 (MAD2) to kinetochores and directs it to function as an inhibitor. MAD1 also helps MAD2 to associate with translocated promoter region (TPr).
外觀
Solution in 0.01 M phosphae buffered saline, pH 7.4, containing 15 mM sodium azide.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Efficient mitotic checkpoint signaling depends on integrated activities of Bub1 and the RZZ complex.
Gang Zhang et al.
The EMBO journal, 38(7) (2019-02-21)
Kinetochore localized Mad1 is essential for generating a "wait anaphase" signal during mitosis, hereby ensuring accurate chromosome segregation. Inconsistent models for the function and quantitative contribution of the two mammalian Mad1 kinetochore receptors: Bub1 and the Rod-Zw10-Zwilch (RZZ) complex exist.
Jun Wan et al.
Current biology : CB, 24(22), 2687-2692 (2014-12-03)
Mitotic arrest deficient 1 (Mad1) plays a well-characterized role in the major cell-cycle checkpoint that regulates chromosome segregation during mitosis, the mitotic checkpoint (also known as the spindle assembly checkpoint). During mitosis, Mad1 recruits Mad2 to unattached kinetochores, where Mad2
Garry G Sedgwick et al.
mAbs, 8(4), 689-697 (2016-03-18)
The spindle assembly checkpoint (SAC) ensures accurate chromosome segregation during mitosis by delaying the activation of the anaphase-promoting complex/cyclosome (APC/C) in response to unattached kinetochores. The Mad2 protein is essential for a functional checkpoint because it binds directly to Cdc20
Gang Zhang et al.
Journal of cell science, 125(Pt 13), 3243-3253 (2012-03-29)
The Ndc80 complex establishes end-on attachment of kinetochores to microtubules, which is essential for chromosome segregation. The Ndc80 subunit is characterized by an N-terminal region that binds directly to microtubules, and a long coiled-coil region that interacts with Nuf2. A
Timo E F Kuijt et al.
Chromosoma, 123(5), 471-480 (2014-04-04)
Fidelity of chromosome segregation is monitored by the spindle assembly checkpoint (SAC). Key components of the SAC include MAD1, MAD2, BUB1, BUB3, BUBR1, and MPS1. These proteins accumulate on kinetochores in early prometaphase but are displaced when chromosomes attach to
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