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Merck

M5814

Sigma-Aldrich

MKK6/SKK3, active human

≥80% (SDS-PAGE), recombinant, expressed in E. coli, Maltose binding protein tagged, buffered aqueous solution

别名:

MAP Kinase Kinase 6, MAP Kinase Kinase 6/SKK 3

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About This Item

MDL號碼:
分類程式碼代碼:
12352200
NACRES:
NA.32

重組細胞

expressed in E. coli

品質等級

化驗

≥80% (SDS-PAGE)

形狀

buffered aqueous solution

分子量

81 kDa

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−70°C

基因資訊

human ... MAP2K6(5608)

特異性

Active in the phosphorylation of p38/SAPK2a

應用

Active enzyme for study of stress-activated protein kinases.
May be used for activation of p38α followed by phosphorylation of MBP.

單位定義

1 unit will activate 1 unit of p38/SAPK2 per minute. 1 unit of active p38/SAPK2 will incorporate 1 nanomole of phosphorus into myelin basic protein per minute.

外觀

Solution in approx. 1 mg/ml in 50 mM Tris-HCl, pH 7.5, 0.1 mM EGTA, 0.1% 2-mercaptoethanol, 0.15 M NaCl, 0.03% Brij® L23, 270 mM sucrose, 0.2 mM PMSF, 1 mM benzamidine.

法律資訊

Brij is a registered trademark of Croda International PLC

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Skin Sens. 1

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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A Cuenda et al.
The EMBO journal, 16(2), 295-305 (1997-01-15)
Stress-activated protein kinase-3 (SAPK3), a recently described MAP kinase family member with a wide-spread tissue distribution, was transfected into several mammalian cell lines and shown to be activated in response to cellular stresses, interleukin-1 (IL-1) and tumour necrosis factor (TNF)

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