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Merck
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文件

L9504

Sigma-Aldrich

D -荧光素

synthetic

别名:

( S )-2-（6-羟基-2-苯并噻唑基）-2-噻唑啉-4-羧酸, 4,5-二氢-2-（6-羟基-2-苯并噻唑基）-4-噻唑羧酸, 萤火虫荧光素

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About This Item

经验公式（希尔记法）:
C11H8N2O3S2
CAS号:
2591-17-5
分子量:
280.32
Beilstein:
30484
EC號碼:
219-981-3
MDL號碼:
MFCD00042929
分類程式碼代碼:
12352204
PubChem物質ID:
24896524
NACRES:
NA.32

生物源

synthetic

品質等級

300

化驗

≥98% (HPLC)

形狀

powder

溶解度

DMSO: 10 mg/mL, clear, colorless to faintly yellow

螢光

λex 328 nm; λem 532 nm in H2O

儲存溫度

−20°C

SMILES 字串

OC(=O)[C@H]1CSC(=N1)c2nc3ccc(O)cc3s2

InChI

1S/C11H8N2O3S2/c14-5-1-2-6-8(3-5)18-10(12-6)9-13-7(4-17-9)11(15)16/h1-3,7,14H,4H2,(H,15,16)/t7-/m1/s1

InChI 密鑰

BJGNCJDXODQBOB-SSDOTTSWSA-N

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一般說明

荧光素是一种大量存在于发光性生物体中的小分子。该分子对氧和光敏感。

應用

荧光素用于通过荧光素酶检测测定小鼠和拟南芥种子中的荧光素酶活性。它也被用于测量新生大鼠心室心肌细胞的生物发光。
Km 约为 2 μM 的萤火虫荧光素酶的底物。

生化／生理作用

细胞内的荧光素摄入受到三磷酸腺苷(ATP)结合盒(ABC)转运体抑制剂的调节，例如ATP结合盒转运子G2 (ABCG2)/乳腺癌耐受蛋白(BCRP)抑制剂烟曲霉毒素C等。这一基质被萤火虫萤光素酶氧化，发出能够穿过活组织的光量子。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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