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产品名称
白血病抑制因子 人, LIF, recombinant, expressed in E. coli, 10 μg/ml, buffered aqueous solution (pH 7.4), suitable for cell culture
重組細胞
expressed in E. coli
品質等級
化驗
≥95% (SDS-PAGE)
形狀
buffered aqueous solution (pH 7.4)
效力
0.1-1.5 ng/mL ED50/EC50
品質
endotoxin tested
技術
cell culture | mammalian: suitable
儲存溫度
2-8°C
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生化／生理作用
白血病抑制因子（LIF）是一种多效性糖蛋白，最初被发现在诱导分化成巨噬细胞时，可抑制小鼠骨髓白血病细胞系M1的增殖。LIF的其他活性后来被陆续发现，人们可能了解其各种同义名，包括DIF、D因子、DIA、DRF、CNDF、HILDA、HSF-III和MLPLI。人LIF通过包含190kDa LIF结合α-链（130kDa，小鼠）和130kDa信号转导β-链（gp130）的受体发挥其作用，其与CNTF、OSM、L-6和IL-11公用同一受体。因此，它是细胞因子受体超家族gp130家族的成员。LIF受体已在几种细胞中得到鉴定，包括单核细胞、肝脏、胎盘和胚胎干细胞。天然LIF是高度糖基化的，研究显示根据来源不同，其分子量为32kDa至62kDa，但是没有糖基化似乎不影响其生物活性。LIF由单个基因编码，在人或小鼠中其分泌为含有180个氨基酸的单链糖蛋白，并具有保守的二硫键。人和小鼠LIF共享78％的序列同源性。人LIF可激活小鼠细胞，但小鼠LIF不能激活人体细胞。
白血病抑制因子（LIF）是一种多效性糖蛋白，最初被发现在诱导分化成巨噬细胞时，可抑制小鼠骨髓白血病细胞系M1的增殖。人和小鼠LIF共享78％的序列同源性。人LIF可激活小鼠细胞，但小鼠LIF不能激活人体细胞。
外觀
溶于含有0.02％吐温® 20的磷酸盐缓冲盐水中的溶液（经 0.2 μm过滤）。
分析報告
使用人白血病细胞系TF-1在培养物中对人LIF的增殖活性进行测定。
法律資訊
TWEEN is a registered trademark of Croda International PLC
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Magda Tomala et al.
Protein expression and purification, 73(1), 51-57 (2010-04-13)
Leukemia inhibitory factor (LIF) is a polyfunctional cytokine with numerous regulatory effects in vivo and in vitro. In stem cell cultures it is the essential media supplement for the maintenance of pluripotency of embryonic and induced pluripotent stem cells. With
N M Gough et al.
Proceedings of the National Academy of Sciences of the United States of America, 85(8), 2623-2627 (1988-04-01)
A human homologue of the recently cloned murine leukemia-inhibitory factor (LIF) gene was isolated from a genomic library by using the murine cDNA as a hybridization probe. The nucleotide sequence of the human gene indicated that human LIF has 78%
Dumrongkiet Arthan et al.
Cancer letters, 297(1), 31-41 (2010-06-24)
Medullary thyroid carcinoma (MTC) is a multiple endocrine neoplasia type 2 syndrome caused by mutations in extracellular receptor or intracellular kinase domains of the RET proto-oncogene. Activation of the Ras/Raf/MEK/ERK pathway can lead to growth arrest by secreting leukemia inhibitory
Borhane Annabi et al.
Journal of neuroinflammation, 6, 8-8 (2009-03-11)
The CD133(+) stem cell population in recurrent gliomas is associated with clinical features such as therapy resistance, blood-brain barrier disruption and, hence, tumor infiltration. Screening of a large panel of glioma samples increasing histological grade demonstrated frequencies of CD133(+) cells
Young Mok Lee et al.
Biology of reproduction, 75(3), 380-386 (2006-05-26)
In this study, we proposed a testis-mediated germline chimera production system based on the transplantation of testicular cells directly into heterologous testes. The testicular cells of juvenile (4-wk-old) or adult (24-wk-old) Korean Ogol chickens with a recessive pigmentation inhibitory gene
商品
Naive pluripotent stem cells are located within the epiblast of mature blastocysts. These primitive “ground-state” cells may be cultured in vitro using specialized media and small molecule inhibitors.
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