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Merck
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文件

L3790

Sigma-Aldrich

Lysozyme 来源于鸡蛋白

10 mg/mL

别名:

粘肽N-乙酰胞壁质水解酶, 胞壁质酶

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About This Item

CAS号:
12650-88-3
EC 号:
3.2.1.17 (BRENDA, IUBMB)
MDL號碼:
MFCD00131557
分類程式碼代碼:
12352204
NACRES:
NA.56

生物源

chicken egg white

品質等級

200

形狀

liquid

分子量

single-chain 14.3 kDa

濃度

≥400000 units/mL
10 mg/mL

技術

cell based assay: suitable

適合性

suitable for cell lysis

UniProt登錄號

P00698

應用

cell analysis

運輸包裝

wet ice

儲存溫度

−20°C

基因資訊

chicken ... LYZ(396218)

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相关类别

一般說明

溶酶体是一种存在于蛋清中的酶，它能分解细菌细胞壁，提高提取 DNA 和蛋白质的效率。

應用

适用于细菌 DNA 和蛋白质的纯化。
酶可分解细菌的细胞壁；用于制备原生质体。

生化／生理作用

溶菌酶可水解肽聚糖中N-乙酰胞壁酸与N-乙酰-D-氨基葡萄糖残基之间以及壳糊精中N-乙酰-D-氨基葡萄糖残基之间的β（1→4）连接。 革兰氏阳性细胞对这种水解非常敏感，因为它们的细胞壁中的肽聚糖比例很高。革兰氏阴性菌就对这种水解没那么敏感，因为它们有外膜并且肽聚糖的比例较低。然而，这些细胞可能在细胞外膜中螯合金属离子的EDTA存在的情况下水解。

该酶在广泛的pH范围（6.0至9.0）内具有活性。pH值为6.2时，可在更广的离子强度范围（0.02至0.100 M）观察到最大活性，而pH值为9.2时为0.01至0.06 M。

適合性

采用 CelLytic ™ B 从细菌中提取蛋白质的适用性试验。

外觀

50% 甘油、25 mM 醋酸钠溶液
即用型溶液：10 mg/mL溶菌酶，溶于含50%甘油的25 mM醋酸钠（pH 4.5）中。

法律資訊

CelLytic is a trademark of Sigma-Aldrich Co. LLC

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H334

防範說明

P261 - P284 - P501

危險分類

Resp. Sens. 1

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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实验方案

Enzymatic Assay of Lysozyme (EC 3.2.1.17)

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

Enzymatic Assay of Lysozyme (EC 3.2.1.17)

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

Plasmid DNA Preparation

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

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