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生物源
Glycine max (soybean)
共軛
peroxidase conjugate
化驗
85.00-100.00% (Warburg-Christian)
形狀
lyophilized powder
效力
<80 μg per mL agglutination activity
peroxidase activity
25-100 units/mg protein
成份
Protein, ~95% modified Warburg-Christian
技術
western blot: suitable
UniProt登錄號
儲存溫度
−20°C
基因資訊
soybean ... LE1(100818710)
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應用
General Western Blot Protocol:
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
生化／生理作用
SBA不是血型特异性的，但对N-乙酰-D-半乳糖胺具有亲和力。
單位定義
One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0, at 20 °C.
外觀
Contains sodium citrate
準備報告
由亲和纯化凝集素制备偶联物。
Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.
分析報告
凝集活性以 μg/ml 表示，并且在浓度为1 mg/ml、pH 6.8的磷酸缓冲盐水中进行连续稀释测定。该活性是在25℃温育1小时后，凝集2％人血型A红细胞悬浮液的最低浓度。
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
C Biel et al.
Archives of toxicology, 96(6), 1815-1827 (2022-04-17)
Human Precision-cut intestinal slices (hPCIS) are used to study intestinal physiology, pathophysiology, drug efficacy, toxicology, kinetics, and metabolism. However, the use of this ex vivo model is restricted to approximately a 24 h timeframe because of declining viability of the hPCIS
A simple method for the preparation of enzyme-antibody conjugates.
M J O'Sullivan et al.
FEBS letters, 95(2), 311-313 (1978-11-15)
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