生物源
mouse
共軛
unconjugated
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
LUC-1, monoclonal
形狀
buffered aqueous solution
分子量
60 kDa
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
濃度
~2 mg/mL
技術
immunocytochemistry: 20-40 μg/mL using transfected 293Tcells expressing luciferase
immunocytochemistry: suitable
western blot: 2-4 μg/mL using whole extract of transfected 293T cells expressing luciferase
western blot: suitable
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
一般說明
小鼠单克隆抗荧光素酶抗体可识别转染细胞中的重组荧光素酶。该抗体可为用户提供荧光素酶的一种替代检测方法。由于它可直接检测荧光素酶蛋白,因此不依赖于荧光素酶活性或快速反应动力学的测量。这还使得原位检测荧光素酶的表达成为可能,并在样品组之间具有可重复性。
小鼠单克隆抗荧光素酶抗体(小鼠IgG1同种型)来源于LUC-1 杂交瘤,其通过融合小鼠骨髓瘤细胞和萤火虫荧光素酶免疫的BALB /c小鼠的脾细胞产生。荧光素酶是一种受体蛋白,含有两个结合区,一个为ATP结合区,另一个为荧光素底物结合区。两个结合区彼此非常接近。
特異性
小鼠单克隆抗荧光素酶抗体可识别转染 (293T) 的真核细胞的重组荧光素酶。
應用
小鼠单克隆抗荧光素酶抗体已被用于:
- 免疫印迹
- 免疫细胞化学
- 免疫组织化学
生化/生理作用
荧光素酶在存在底物荧光素以及 Mg2+、氧和ATP的情况下催化生物荧光反应。荧光素酶检测非常迅速、灵敏,而且和CAT检测不同,不需要反射性底物。抗荧光素酶抗体可以原位检测荧光素酶表达,提供了一种研究信号序列激活中细胞定位的方法。
外觀
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
準備報告
工作稀释度:1-2 μg/ml
儲存和穩定性
如需连续使用,可在2-8 °C保存至多一月。 若需延长储存时间,可将溶液分装并冷冻。 不建议反复冻融或存放在“无霜”冰柜中。 如果长期储存后发生轻微浑浊,请在使用前通过离心澄清溶液。 如果在12小时内未使用,则应丢弃工作稀释样品。
免責聲明
除非我们的产品目录另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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