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應用
用于纯化含有（D-N-乙酰氨基葡萄糖）2或N-乙酰神经氨酸的糖蛋白。
生化／生理作用
WGA 不具有血型特异性，但具有与 N-乙酰基-β-D-葡糖胺残基和 N-乙酰基-β-D-葡糖胺低聚物的亲和性。WGA 不含蛋白结合糖。
外觀
含15 mM叠氮化钠的0.9% NaCl中的混悬剂。
準備報告
由亲和纯化凝集素制备偶联物。
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
其他客户在看
Bo F Hansen et al.
PloS one, 7(5), e34274-e34274 (2012-05-17)
There is controversy with respect to molecular characteristics of insulin analogues. We report a series of experiments forming a comprehensive characterisation of the long acting insulin analogues, glargine and detemir, in comparison with human insulin, IGF-1, and the super-mitogenic insulin
Liheng Yang et al.
The Journal of biological chemistry, 291(8), 3905-3917 (2015-12-20)
The normal cellular prion protein (PrP) is a glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein. However, in pancreatic ductal adenocarcinoma cell lines, such as BxPC-3, PrP exists as a pro-PrP retaining its glycosylphosphatidylinositol (GPI) peptide signaling sequence. Here, we report the identification
D L Christie et al.
The Biochemical journal, 270(1), 57-61 (1990-08-15)
Chromaffin-granule membranes were separated into insoluble and soluble fractions after extraction with lithium di-iodosalicylate (LDIS). These fractions were characterized by one- and two-dimensional gel electrophoresis, and glycoproteins were detected after electroblotting with peroxidase-labelled concanavalin A and wheat-germ agglutinin (WGA). The
María-Ángeles Cortés-Gómez et al.
International journal of molecular sciences, 24(2) (2023-01-22)
In Alzheimer's disease (AD), the reduction in acetylcholinesterase (AChE) enzymatic activity is not paralleled with changes in its protein levels, suggesting the presence of a considerable enzymatically inactive pool in the brain. In the present study, we validated previous findings
Cynthia Tang et al.
Communications biology, 4(1), 161-161 (2021-02-07)
Mutations in CLN3 lead to photoreceptor cell loss in CLN3 disease, a lysosomal storage disorder characterized by childhood-onset vision loss, neurological impairment, and premature death. However, how CLN3 mutations cause photoreceptor cell death is not known. Here, we show that
实验方案
This page shows how to purify glycoproteins and polysaccharides by affinity chromatography using Agarose Wheat Germ Lectin from GE Healthcare.
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