I8523

Interleukin-12 from mouse

≥97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

• 别名: IL-12
• MDL號碼: MFCD00212975
• 分類程式碼代碼: 12352202
• NACRES: NA.32

属性

• 生物源: mouse
• 品質等級: 100
• 重組細胞: expressed in baculovirus infected Sf21 cells
• 化驗: ≥97% (SDS-PAGE)
• 形狀: lyophilized powder
• 效力: 0.01-.1 ng/mL ED₅₀
• 分子量: glycoprotein 70 kDa
• 包裝: pkg of 10 μg
• 技術: cell culture | mammalian: suitable
• 雜質: endotoxin, tested
• UniProt登錄號: P43431, P43432
• 儲存溫度: −20°C
• 基因資訊: mouse ... Il12a(16159) , Il12b(16160)

说明

應用

Interleukin-12 (IL-12) from mouse has been used to study the effect of IL-12 on Th1 (type 1 T helper) immunity in Mongolian gerbils (Meriones unguiculatus) infected with Sporothrix schenckii. It has been used as a standard in ELISA (enzyme-linked immunosorbent assay) cytokine assay.

生化／生理作用

Interleukin-12 (IL-12) was identified as a factor secreted by human Epstein-Barr (EBV)-transformed B cell lines. IL-12, or Natural Killer Cell Stimulatory Factor (NKSF), is a 75 kDa disulfide-linked heterodimer of a 35 kDa subunit and 40 kDa subunit. IL-12 is produced predominantly by monocytes and NK cells and induces T cells and NK cells to produce IFN-γ. Human IL-12 is not active on mouse cells, but murine IL-12 is active on both murine and human lymphocytes. The biological activity of recombinant human IL-12 was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. IL-12 has anti-angiogenic properties.

外觀

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, type N95 (US)