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Key Documents

GERPN2134

ECL Western Blotting Reagents

Cytiva RPN2134

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About This Item

分類程式碼代碼:
41105332
NACRES:
NA.32

特點

wetted part: no

包裝

pkg of 1 kit

製造商/商標名

Cytiva RPN2134

顏色

Detection reagent 1: Colorless Detection reagent 2: Colorless

儲存溫度

2-8°C

一般說明

Amersham ECL Western Blotting Detection Reagents were the world′s first commercial chemiluminescent reagents for Western blotting and are still the most widely used. They are at least 10-fold more sensitive than colorimetric assays and produce excellent and reliable results every time.

These reagents use enhanced luminol-based detection which is suitable for all routine western blotting. ECL Western Blotting Detection Reagents in combination with Hybond® ECL membranes form a complete solution for Western blot applications.

Contents include the following:
GERPN2109, 62.5 mL each of ECL Detection Reagents 1 and 2 (1000 cm2)
GERPN2209, 125 mL each (2000 cm2)
GERPN2106, 250 mL each (4000 cm2)
GERPN2134, 375 mL each (6000 cm2)

應用

ECL Western Blotting Reagents have been used in Western blotting.

特點和優勢

  • High sensitivity, ~10 pg in model system.
  • Proven performance, the most frequently cited ECL Western Blotting Detection Reagents in publications
  • Detectable on Hyperfilm ECL or with CCD imager
  • Recommended membrane Hybond ECL

成分

The kit contains
3 x 125 mL of ECL Detection reagent 1
3 x 125 mL of ECL detection reagent 2

Sufficient for detection of 6000 cm2 of membrane:
375 mL Detection reagent 1(color : colorless)
375 mL Detection reagent 2 (color: colorless)

儲存和穩定性

Please be aware this product may be shipped 30 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Amersham is a trademark of Cytiva
ECL is a trademark of Cytiva
Hybond is a registered trademark of Cytiva

儲存類別代碼

10 - Combustible liquids


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Troubleshooting of Detection Methods of GST-tagged Proteins

实验方案

This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from GE Healthcare.

This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using GE Healthcare products.

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