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TALON® Superflow Cobalt-IMAC
製造商/商標名
Cytiva 29-0005-93
包裝
pkg of 50 x 100 μL
床體積
100 μL
基質
6% cross-linked agarose
粒徑
60-160 μm
cleaning
2-14(Co2+-stripped medium)
工作範圍
3-12(Co2+-stripped medium)
容量
≤1 mg binding capacity(histidine-tagged)
≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)
儲存溫度
2-8°C
一般說明
His SpinTrap™ Talon® is a single-use column for rapid screening with small samples of histidine-tagged proteins.
應用
His SpinTrap™ Talon® is designed for high purity, small-scale purification and screening of histidine-tagged Proteins from clarified cell lysates. His SpinTrap™ Talon® columns are used together with a standard microcentrifuge and purification takes approximately 10 minutes. The columns make parallel preparations of few or many small-scale cell lysates quick and easy. Prepacked columns enable highly reproducible column to column purification and yield, with RSD < 6%. Leakage of cobalt ions from Talon® Superflow medium is negligible.
特點和優勢
- Suitable for purification of multiple samples in parallel, such as screening.
- Convenient, ready-to-use single-use spin column, prepacked with 100 μL Talon® Superflow cobalt-IMAC (immobilized metal affinity chromatography) medium offering a different selectivity compared to nickel-charged media.
- With a microcentrifuge, one run takes about 10 minutes.
儲存和穩定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析報告
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律資訊
SpinTrap is a trademark of Cytiva
Talon is a registered trademark of Clontech
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
商品
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.
实验方案
This page shows how to purify histidine-tagged proteins using TALON Superflow from GE Healthcare.
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