推荐产品
材料
bed support (polyamide)
glass column
glass fiber (polypropylene reinforced)
分子量
10000-600000 Da (globular proteins)
包裝
pkg of 1 ea
製造商/商標名
Cytiva 28-9893-35
參數
<1.0 mL/min flow rate
<5 mL sample volume
0.3 Mpa (42 psi)
床體積
120 mL
柱長度 × 內徑
60 cm × 16 mm
基質
agarose dextran composite
dextran-agarose particle platform
polymeric
基質活性組
dextran-agarose phase
樣品平均尺寸
34 μm
cleaning
1-14
工作範圍
3-12
分離技術
size exclusion (SEC)
一般說明
Superdex®制备级(pg)是通过葡聚糖与高度交联琼脂糖的共价结合产生的。这些介质的分离性质由葡聚糖组分决定。陡峭的选择性曲线为分子量范围内的多肽和蛋白质提供了优良的分辨能力:Mr < 10 000 (Superdex® 30 pg)、Mr 3 000至70 000 (Superdex® 75 pg)以及Mr 10 000 600 000 (Superdex® 200 pg)。该培养基结合了高机械强度和高亲水性,允许高流速和极低的非特异性相互作用。
特點和優勢
- 分辨率高,运行时间短,回收率良好。
- 高流量下的高容量。
- 分离可以扩展至生产水平。
- 预先包装实现了方便和再现性。
- 1/16”配件方便连接到AKTA设计系统。
適合性
儲存和穩定性
分析報告
法律資訊
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
商品
This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from GE Healthcare.
Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.
Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.
This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.
实验方案
This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap Protein G HP from GE Healthcare for the capture step.
AC separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. With such high selectivity and hence high resolution for the protein(s) of interest, purification levels in the order of several thousand-fold with high recovery of active material are achievable.
This page shows how to refold proteins using gel filtration and products from GE Healthcare.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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