跳转至内容
Merck
所有图片(1)

Key Documents

GE28-9523-59

GST Spintrap

Cytiva 28-9523-59

登录查看公司和协议定价


About This Item

分類程式碼代碼:
41106500
NACRES:
NA.56

ligand

glutathione

包裝

pkg of 50 ea

製造商/商標名

Cytiva 28-9523-59

床體積

50 μL

基質

4% agarose

粒徑

45-165 μm

平均直徑

90 μm

cleaning

4-13

工作範圍

4-13

容量

>25 mg binding capacity (horse liver GST/ml medium)
≥500 μg binding capacity(recombinant glutathione S-transferase (rGST))

一般說明

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
GST SpinTrap columns are designed for rapid small-scale purification of GST-tagged proteins directly from clarified or unclarified cell lysates. GST-tagged proteins can be recovered under mild elution conditions (10 mM glutathione), which preserves the functionality of the proteins, and more than 90% purity can be obtained in a single step. GST SpinTrap columns are suitable for purification of multiple samples in parallel, for example in screening experiments such as evaluation of chromatography media, growth temperature, culture density, and other variables to find optimal conditions for expression.

特點和優勢

  • Preserves protein antigenicity and function.
  • Yields up to 500 μg of pure protein per column.
  • Allows direct purification of both clarified and unclarified cell lysates.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (PBS and 0,05% Kathon CG/ICP Biocide)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

SpinTrap is a trademark of Cytiva

儲存類別代碼

12 - Non Combustible Liquids


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

商品

This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.

This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.

This page describes characteristics of Glutathione Sepharose and HiTrap Benzamidine FF (high sub) media and columns from GE Healthcare.

This page describes troubleshooting strategies in purification of GST-tagged proteins using GE Healthcare products.

实验方案

This page how to remove GST tags by enzymatic cleavage using GE Healthcare products.

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from GE Healthcare.

Characteristics of Glutathione Sepharose® Products

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门