推荐产品
ligand
concanavalin A (con A)
包裝
pack of 5 × 1 mL
製造商/商標名
Cytiva 28-9520-85
儲存條件
(0.1 M acetate buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl, 20% Ethanol)
(20% Ethanol containing 0.1 M Acetate Buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl2)
參數
42 psi
床尺寸
7 mm × 25 mm
床體積
1 mL
柱內徑
7 mm
基質
4% agarose
粒徑
45-165 μm
平均直徑
90 μm
cleaning
4-9
工作範圍
4-6
儲存溫度
2-8°C
一般說明
HiTrap® Con A 4B is a ready-to-use column prepacked with Con A Sepharose™ 4B, a medium for convenient separation and purification of glycoproteins, polysaccharides, and glycolipids.
應用
Concanavalin A (Con A) is a tetrameric metalloProtein isolated from Canavalia ensiformis (jack bean). Con A binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl, and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose™ is routinely used for separation and purification of glycoProteins, polysaccharides, and glycolipids.
特點和優勢
- Con A Sepharose™ is concanavalin A coupled to Sepharose™ 4B.
- HiTrap® Con A 4B columns can be operated with a syringe, a peristaltic pump, or a liquid chromatography system such as ÄKTA® design.
儲存和穩定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析報告
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律資訊
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
实验方案
This page shows how to purify or remove glycoproteins and polysaccharides with Con A Sepharose 4B, Lentil Lectin Sepharose 4B, Capto Lentil Lectin from GE Healthcare.
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
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