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Key Documents

GE28-9520-85

HiTrap® Con A 4B

Cytiva 28-9520-85, pack of 5 × 1 mL

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About This Item

分類程式碼代碼:
23151816
NACRES:
NA.56

ligand

concanavalin A (con A)

包裝

pack of 5 × 1 mL

製造商/商標名

Cytiva 28-9520-85

儲存條件

(0.1 M acetate buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl, 20% Ethanol)
(20% Ethanol containing 0.1 M Acetate Buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl2)

參數

42 psi

床尺寸

7 mm × 25 mm

床體積

1 mL

柱內徑

7 mm

基質

4% agarose

粒徑

45-165 μm

平均直徑

90 μm

cleaning

4-9

工作範圍

4-6

儲存溫度

2-8°C

一般說明

HiTrap® Con A 4B is a ready-to-use column prepacked with Con A Sepharose 4B, a medium for convenient separation and purification of glycoproteins, polysaccharides, and glycolipids.

應用

Concanavalin A (Con A) is a tetrameric metalloProtein isolated from Canavalia ensiformis (jack bean). Con A binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl, and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose is routinely used for separation and purification of glycoProteins, polysaccharides, and glycolipids.

特點和優勢

  • Con A Sepharose is concanavalin A coupled to Sepharose 4B.
  • HiTrap® Con A 4B columns can be operated with a syringe, a peristaltic pump, or a liquid chromatography system such as ÄKTA® design.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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实验方案

This page shows how to purify or remove glycoproteins and polysaccharides with Con A Sepharose 4B, Lentil Lectin Sepharose 4B, Capto Lentil Lectin from GE Healthcare.

Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.

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