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Key Documents

GE28-9505-13

HiScreen SP Fast Flow

Cytiva 28-9505-13

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About This Item

分類程式碼代碼:
47101511
NACRES:
NA.56

ligand

sulphopropyl

描述

Ion Exchanger Type (value)

包裝

pkg of 1 ea

製造商/商標名

Cytiva 28-9505-13

參數

1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi

床尺寸

7.7 mm × 100 mm

床體積

4.7 mL

柱內徑

7.7 mm

基質

6% cross-linked agarose

粒徑

45-165 μm

平均直徑

90 μm

cleaning

3-14

工作範圍

4-13

適合性

suitable for bioprocess medium

一般說明

HiScreen SP FF columns are prepacked with SP Sepharose Fast Flow, a strong cation exchanger, and are part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening.

應用

The small column volume of 4.7 mL and bed height of 10 cm make HiScreen columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up. If necessary, two columns can easily be connected in series to give a bed height of 20 cm. Media prepacked in HiScreen IEX FF columns are also available in other formats and as bulk packs, making it possible to use the same media for development work, pilot studies, and routine production.

特點和優勢

  • SP Sepharose Fast FLow is a strong cation exchanger and IEX Sepharose Fast FLow medium are well-proven, industrial standards for ion exchange chromatography.
  • Prepacked, ready-to-use columns for convenient process development optimization.
  • 10 cm bed height of HiScreen columns is designed to allow method optimization and parameter screening.
  • Easily connected in series to achieve 20 cm bed height.
  • Small bed volume gives fast results and minimal sample/buffer consumption.
  • Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)
Store at 4 to 30 °C (20% Ethanol, 0.2 M Sodium Acetate)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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商品

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using GE Healthcare products.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

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实验方案

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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