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Key Documents

GE28-9356-04

Sigma-Aldrich

HiPrep 16/60 Sephacryl® S-400 HR

Cytiva 28-9356-04, column L × I.D. 60 cm × 16 mm, 50 μm avg. part. size

别名:

HiPrep Chromatography Column, Sephacryl S-400 HR

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About This Item

分類程式碼代碼:
41115700
NACRES:
SB.52

材料

polypropylene (PP)

分子量

20000-8000000 Da (globular proteins)

包裝

pkg of 1 ea

製造商/商標名

Cytiva 28-9356-04

參數

<0.5 mL/min flow rate
<5 mL sample volume
0.15 Mpa (22 psi)

床體積

120 mL

柱長度 × 內徑

60 cm × 16 mm

基質

dextran-acrylamide particle platform
allyl dextran and N, N’-methylenebisacrylamide
polymeric

基質活性組

dextran-acrylamide phase

樣品平均尺寸

50 μm

cleaning

2-13

工作範圍

3-11

分離技術

size exclusion (SEC)

一般說明

HiPrep 16/60 Sephacryl® S-400 HR is a prepacked gel filtration column for preparative separation of biomolecules in the molecular weight range of 20 000 to 8 000 000 with sample volumes up to 5 mL.

應用

Sephacryl® S-400 is optimized for separating polysaccarides and other macromolecules with extended structures e.g. proteoglycans and liposomes.

特點和優勢

  • Gel filtration columns for low to medium pressure AKTA systems.
  • The HiPrep Sephacryl® series offer reproducible separations over a wide molecular weight range.
  • Convenient, prepacked HiPrep Sephacryl® columns speed up protein purification.
  • Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/ bisacrylamide matrix.

適合性

All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)
gel filtration columns for low to medium pressure ÄKTA systems+AE14

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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法律資訊

HiPrep is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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