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Key Documents

GE17-5130-01

GSTrap™ Fast Flow

Cytiva 17-5130-01, pack of 5 × 1 mL

别名:

GSTrap column

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About This Item

分類程式碼代碼:
23151817
NACRES:
NA.25

ligand

glutathione and 10-carbon linker arm

包裝

pack of 5 × 1 mL

製造商／商標名

Cytiva 17-5130-01

參數

flow rate
42 psi

床尺寸

7 mm × 25 mm

床體積

1 mL

柱內徑

7 mm

基質

highly cross-linked 4% agarose

平均直徑

90 μm

cleaning

3-12

工作範圍

3-12

容量

>10 mg/mL binding capacity

一般說明

GSTrap™ FF columns are prepacked Glutathione Sepharose™ Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.

GST*-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap™ FF.GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose™ 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as AKTA design or FPLC™ System.

* See the section for licensing information in our terms and conditions.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

特點和優勢

  • Fast and simple one-step purification of GST-tagged proteins.
  • Mild elution conditions preserving protein antigenicity and function.
  • Easy scale-up by connecting the columns in series.
  • Sepharose™ 4 Fast Flow provides good flow properties.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

FPLC is a trademark of Cytiva
GSTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

分析证书(COA)

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商品

Troubleshooting of Cleavage Methods

This page shows troubleshooting strategies for cleavage methods using GE Healthcare products.

Cleavage and Purification of GST-Tagged Protein Eluted from GSTrap

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from GE Healthcare.

Troubleshooting Purification Methods

This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.

Troubleshooting Purification Methods

This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.

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实验方案

Removal of Thrombin and Factor Xa Using HiTrap® Benzamidine FF (High Sub)

This page shows how to remove thrombin and Factor Xa using HiTrap® Benzamidine FF (high sub) from GE Healthcare.

Removal of GST Tag by Enzymatic Cleavage

This page how to remove GST tags by enzymatic cleavage using GE Healthcare products.

Performing a Separation with GE Healthcare Products Based on GST Fusion Proteins

How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from GE Healthcare.

Purification Using GSTrap™ HP, GSTrap™ FF, and GSTrap™ 4B columns

This page shows how to purify GST-tagged proteins using GSTrap HP, GSTrap FF and GSTrap 4B columns from GE Healthcare.

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