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Key Documents

GE17-5113-01

Streptavidin Sepharose High Performance

Cytiva 17-5113-01, pack of 5 mL

别名:

affinity chromatography medium, high performance affinity resin, biotinylated proteins purification

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About This Item

分類程式碼代碼:
41106500
NACRES:
NA.56

包裝

pack of 5 mL

製造商/商標名

Cytiva 17-5113-01

儲存條件

(20% Ethanol)

基質

highly cross-linked 6% agarose

樣品平均尺寸

34 μm

cleaning in place

2-10.5

工作範圍

4-9

容量

binding capacity(>300 nmol biotin/ml medium 6 mg biotinylated BSA/ml medium)

儲存溫度

2-8°C

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一般說明

Streptavidin Sepharose High Performance is an affinity chromatography medium designed for fast and reliable, high resolution purification of biotinylated biomolecules.

Purified Streptavidin isolated from Streptomyces avidinii is immobilised on Sepharose High Performance. The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability. The immobilised streptavidin binds biotin and biotinylated substances through affinity chromatography.

特點和優勢

  • Streptavidin immobilized to Sepharose High Performance for purification of biotinylated compounds
  • Extremely useful for exploiting either the strong interactions of biotin and streptavidin or the somewhat weaker interactions of 2-iminobiotin and streptavidin.
  • 34 μm bead size for improved resolution

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Sepharose is a trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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Jérôme Zervudacki et al.
The EMBO journal, 37(14) (2018-06-07)
Mobilization of transposable elements (TEs) in plants has been recognized as a driving force of evolution and adaptation, in particular by providing genes with regulatory modules that impact their transcription. In this study, we employed an ATCOPIA93 long-terminal repeat (LTR)
Hana Antonicka et al.
Cell metabolism, 32(3), 479-497 (2020-09-03)
We used BioID, a proximity-dependent biotinylation assay with 100 mitochondrial baits from all mitochondrial sub-compartments, to create a high-resolution human mitochondrial proximity interaction network. We identified 1,465 proteins, producing 15,626 unique high-confidence proximity interactions. Of these, 528 proteins were previously
Sarah van Veen et al.
Nature, 578(7795), 419-424 (2020-01-31)
ATP13A2 (PARK9) is a late endolysosomal transporter that is genetically implicated in a spectrum of neurodegenerative disorders, including Kufor-Rakeb syndrome-a parkinsonism with dementia1-and early-onset Parkinson's disease2. ATP13A2 offers protection against genetic and environmental risk factors of Parkinson's disease, whereas loss

商品

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

实验方案

This page shows how to purify or remove biotin and biotinylated substances with Streptavidin Sepharose High Performance from GE Healthcare.

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.

This page shows how to prepare samples for purification with affinity chromatography.

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