推荐产品
ligand
2-mercaptopyridine
包裝
pack of 1 × 5 mL
製造商/商標名
Cytiva 17-5111-01
儲存條件
(20% Ethanol)
參數
<20 mL/min flow rate
42 psi
床尺寸
16 mm × 25 mm
床體積
5 mL
柱內徑
16 mm
基質
highly cross-linked 6% agarose
樣品平均尺寸
34 μm
cleaning
2-13
工作範圍
3-11
儲存溫度
2-30°C
一般說明
HiTrap® IgY Purification HP is a 5 mL column prepacked with Sepharose™ High Performance for fast and easy purification of IgY from egg yolk.
應用
HiTrap® IgY Purification HP is packed with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose™ High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgY and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.
特點和優勢
- Each column binds IgY from a one-quarter portion of egg yolk (approx. 100 mg IgY). For greater capacity, several columns may be connected in series.
- Packed with a thiophilic adsorption medium, the ligand, 2-merCaptopyridine is coupled to Sepharose™ High Performance.
- Recovery and purity of IgY are excellent.
- Can be operated with a liquid chromatography system such as ÄKTA® design.
- The top and bottom frits are manufactured from porous polyethylene
- Connectors for usage with different chromatography systems and other equipment are included
儲存和穩定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析報告
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
其他說明
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
法律資訊
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
商品
This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
实验方案
This page shows how to prepare samples for purification with affinity chromatography.
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