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Key Documents

GE17-5111-01

HiTrap® IGY Purification

Cytiva 17-5111-01, pack of 1 × 5 mL

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About This Item

分類程式碼代碼:
23151816
NACRES:
NA.56

ligand

2-mercaptopyridine

包裝

pack of 1 × 5 mL

製造商/商標名

Cytiva 17-5111-01

儲存條件

(20% Ethanol)

參數

<20 mL/min flow rate
42 psi

床尺寸

16 mm × 25 mm

床體積

5 mL

柱內徑

16 mm

基質

highly cross-linked 6% agarose

樣品平均尺寸

34 μm

cleaning

2-13

工作範圍

3-11

儲存溫度

2-30°C

一般說明

HiTrap® IgY Purification HP is a 5 mL column prepacked with Sepharose High Performance for fast and easy purification of IgY from egg yolk.

應用

HiTrap® IgY Purification HP is packed with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgY and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.

特點和優勢

  • Each column binds IgY from a one-quarter portion of egg yolk (approx. 100 mg IgY). For greater capacity, several columns may be connected in series.
  • Packed with a thiophilic adsorption medium, the ligand, 2-merCaptopyridine is coupled to Sepharose High Performance.
  • Recovery and purity of IgY are excellent.
  • Can be operated with a liquid chromatography system such as ÄKTA® design.
  • The top and bottom frits are manufactured from porous polyethylene
  • Connectors for usage with different chromatography systems and other equipment are included

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他說明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律資訊

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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