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Key Documents

GE17-5054-01

HiTrap® SP Fast Flow

Cytiva 17-5054-01, pack of 5 × 1 mL

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About This Item

分類程式碼代碼:
23151816
NACRES:
NA.56

ligand

sulphopropyl

描述

Ion Exchanger Type (value)

包裝

pack of 5 × 1 mL

製造商/商標名

Cytiva 17-5054-01

參數

flow rate
42 psi

床尺寸

7 mm × 25 mm

床體積

1 mL

柱內徑

7 mm

基質

6% cross-linked agarose

粒徑

45-165 μm

平均直徑

90 μm

cleaning

3-14

工作範圍

4-13

適合性

suitable for bioprocess medium

一般說明

HiTrap® SP FF is prepacked with SP Sepharose Fast Flow, and is a strong cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.

應用

Q, SP, DEAE, and CM Sepharose Fast FLow are based on a robust, 6% highly cross-linked beaded Agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast FLow (high sub) is based on 4% highly cross-linked beaded Agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass Proteins.

特點和優勢

  • Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
  • The industry standard for ion exchange chromatography.
  • High fLow rates and good scale-up potential.
  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
  • Predictable scale-up

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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商品

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

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实验方案

This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from GE Healthcare and how to analyze the refolding process with Superdex columns from GE Healthcare.

This page covers the use of Sepharose Fast Flow for purification of proteins.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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