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ligand
recombinant protein G lacking albumin-binding region
包裝
pkg of 1 kit
製造商／商標名
Cytiva 17-1128-01
儲存條件
(20% Ethanol)
參數
<4 mL/min flow rate
42 psi
床尺寸
7 mm × 25 mm
床體積
1 mL
柱內徑
7 mm
基質
highly cross-linked 6% agarose
樣品平均尺寸
34 μm
cleaning
2-9
工作範圍
3-9
容量
>1 mg binding capacity (lgG/column)
儲存溫度
2-8°C
一般說明
MAbTrap® is an affinity chromatography kit, with everything required for small-scale IgG purification from ascites fluid, serum, and cell culture supernatants.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
應用
MAbTrap® Kit is an affinity chromatography kit for fast and effective purification of monoclonal and polyclonal IgG from ascites fluid, serum, and cell culture supernatants. MAbTrap® Kit contains everything necessary to perform a complete one step purification without using complicated equipment. It also eliminates time-consuming buffer preparation and column packing. The kit includes one HiTrap® column, prepacked with Protein G Sepharose™ High Performance.
特點和優勢
- Convenient kit for purification of IgG from monoclonal and polyclonal material using a syringe in less than 15 min.
- Kit contains one HiTrap® Protein G HP column prepacked with Protein G Sepharose™ High Performance and enough buffer concentrate for 20 preparations with a syringe.
- MAbTrap® Kit contains everything necessary to perform a complete one-step purification without using complex equipment.
儲存和穩定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析報告
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
其他說明
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
法律資訊
HiTrap is a registered trademark of Cytiva
MAbTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
商品
This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from GE Healthcare.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
实验方案
This page shows how to prepare samples for purification with affinity chromatography.
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