推荐产品
ligand
porcine heparin
包裝
pack of 50 mL
製造商／商標名
Cytiva 17-0998-01
基質
6% cross-linked agarose
粒徑
45-165 μm
平均直徑
90 μm
cleaning in place
4-13
工作範圍
4-12
適合性
suitable for bioprocess medium
一般說明
Heparin Sepharose™ 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.
Heparin Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified sodium heparin. Immobilization is performed using chemically stable and oriented coupling giving high binding capacity. The chemical stability of the media is solely dictated by the heparin ligand.
This medium allows for fast and reliable separations of biomolecules with an affinity for heparin, including antithrombin III, coagulation factors and other plasma proteins. Additional applications include DNA binding proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors.
Heparin Sepharose™ 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, Heparin Sepharose™ 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
特點和優勢
- Industrial purification of antithrombin III, coagulation factors and other plasma proteins
- Coupling chemistry gives chemically-stable ligand attachment. Stability of medium solely dictated by heparin ligand.
- BioProcess medium supported for industrial applications and well-established in approved processes
儲存和穩定性
分析報告
法律資訊
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
商品
This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from GE Healthcare.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
实验方案
DNA binding proteins form an extremely diverse class of proteins sharing a single characteristic, their ability to bind to DNA. Functionally the group can be divided into those responsible for the replication and orientation of the DNA such as histones, nucleosomes and replicases and those involved in transcription such as RNA/DNA polymerases, transcriptional activators and repressors and restriction enzymes.
How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from GE Healthcare.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.
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