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Key Documents

GE17-0920-03

HiTrap® IMAC High Performance

Cytiva 17-0920-03, pack of 5 × 1 mL

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About This Item

分類程式碼代碼:
23151816
NACRES:
NA.56

包裝

pack of 5 × 1 mL

製造商/商標名

Cytiva 17-0920-03

參數

42 psi

床尺寸

7 mm × 25 mm

床體積

1 mL

柱內徑

7 mm

基質

highly cross-linked 6% agarose

樣品平均尺寸

34 μm

cleaning

2-14(Medium uncharged with metal ions.)

工作範圍

3-12

容量

≥40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

一般說明

HiTrap® IMAC HP is prepacked with IMAC Sepharose High Performance. The medium is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent high-resolution purification of polyhistidine tagged proteins.

應用

IMAC Sepharose HP is supplied free of metal ions. It is charged by the user with the transition metal ion of choice (e.g. Cu2+, Zn2+, Ni2+, or Co2+); these metal ions will bind to the covalently immobilized chelating ligand on the Sepharose.

特點和優勢

  • For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion.
  • Conveniently charge with your metal of choice.
  • Small bead size for high-performance purification.
  • High binding capacity.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他說明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律資訊

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


分析证书(COA)

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商品

This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.

实验方案

This page shows how to purify histidine-tagged proteins using HiTrap IMAC HP and HiTrap IMAC FF from GE Healthcare.

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

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