推荐产品
ligand
N-hydroxysuccinimide activated groups
包裝
pack of 25 mL
製造商/商標名
Cytiva 17-0906-01
儲存條件
(100% Isopropanol)
基質
4% cross-linked agarose
粒徑
45-165 μm
平均直徑
90 μm
cleaning in place
3-13(Ligand dependent)
ligand dependent
3-13
工作範圍
3-12(3 (ligand dependent)-12 (ligand dependent))
適合性
suitable for bioprocess medium
儲存溫度
2-8°C
一般說明
NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups.
This resin provides a spacer arm to the coupled ligand and is therefore particularly suitable for immobilising smaller protein and peptide ligands.
NHS-activated Sepharose™ 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, NHS-activated Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
特點和優勢
- NHS pre-activated medium for coupling of small amino-containing proteins and peptides in process-scale applications
- High level of pre-activation gives a high degree of substitution of the selected ligand
- NHS coupling method results in chemically stable ligand attachment
- BioProcess medium supported for industrial applications
儲存和穩定性
分析報告
法律資訊
訊號詞
Danger
危險聲明
儲存類別代碼
3 - Flammable liquids
商品
This page describes efficient column packing and preparation for affinity chromatography of antibodies.
This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.
实验方案
This page shows coupling through the primary amine of a ligand with a NHS-activated Sepharose High Performance, NHS-activated Sepharose 4 Fast Flow and CNBr-activated Sepharose from Cytiva.
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
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