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Key Documents

GE17-0404-03

HiTrap® Protein G High Performance

Cytiva 17-0404-03, pack of 2 × 1 mL

别名:

HiTrap Protein G, Protein G affinity column

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About This Item

分類程式碼代碼:
23151816
NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

形狀

prepacked column

交聯

cross-linked

包裝

pack of 2 × 1 mL

製造商/商標名

Cytiva 17-0404-03

儲存條件

(20% Ehtanol)
(20% Ethanol)

參數

42 psi

技術

liquid chromatography (LC): suitable

床尺寸

7 mm × 25 mm

床體積

1 mL

柱內徑

7 mm

尺寸

基質

(cross-linked agarose, spherical)

基質活性組

Protein G

樣品平均尺寸

34 μm

cleaning

2-9

工作範圍

3-9

容量

>1 mg binding capacity (lgG/column)
25 mg binding capacity(human IgG/column)

分離技術

affinity

儲存溫度

2-8°C

一般說明

HiTrap® Protein G HP columns are prepacked with Protein G Sepharose High Performance for fast, easy separations of IgG from different species and subclasses.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

特點和優勢

  • High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
  • 34 μm bead size for high performance, high-resolution purifications.
  • Binds broad range of different species and subclasses of IgG.
  • Simple and proven method giving reproducible results.
  • Designed for use with syringe, peristalic pump, or chromatography system.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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Nimitha R Mathew et al.
Cell reports, 35(12), 109286-109286 (2021-06-24)
B cell responses are critical for antiviral immunity. However, a comprehensive picture of antigen-specific B cell differentiation, clonal proliferation, and dynamics in different organs after infection is lacking. Here, by combining single-cell RNA and B cell receptor (BCR) sequencing of

商品

This page describes automated purification of antibodies using ÄKTA chromatography systems.

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from GE Healthcare.

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

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实验方案

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap Protein G HP from GE Healthcare for the capture step.

This page shows how to prepare samples for purification with affinity chromatography.

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