跳转至内容
Merck
所有图片(3)

主要文件

GE17-0402-03

HiTrap® Protein A High Performance

Cytiva 17-0402-03, pack of 2 × 1 mL

登录查看公司和协议定价
所有图片(3)

About This Item

分類程式碼代碼:
23151816
NACRES:
NA.56

ligand

native protein A

包裝

pack of 2 × 1 mL

製造商／商標名

Cytiva 17-0402-03

儲存條件

(20% Ethanol)

參數

42 psi

床尺寸

7 mm × 25 mm

床體積

1 mL

柱內徑

7 mm

尺寸

樣品平均尺寸

34 μm

cleaning

2-10

工作範圍

3-9

儲存溫度

2-8°C

一般說明

HiTrap® protein A HP is a prepacked protein A Sepharose™ High Performance column for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

應用

HiTrap® Protein A HP is a prepacked ready to use, column for preparative purification of monoclonal and polyclonal antibodies. The special design of the column, together with the medium provide fast, simple and easy separations in a convenient format.

特點和優勢

  • High binding capacitiy of 20 mg IgG/mL medium and fast kinetics.
  • 34 μm bead size for high-performance, high-resolution purifications.
  • Designed for use with syringe, pump, or chromatography system such as ÄKTA® design or FPLC™ System.
  • Stable over pH 3 to 9 for use, and 2 to 10 for cleaning.
  • Robust, easy-to-use method.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 文件 section.

如需帮助，请联系 客户支持

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

商品

Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP
IgG Antibody Purification with HiTrap® Protein G HP Columns

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from GE Healthcare.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

实验方案

Performing a Separation with GE Healthcare Products Based on Protein A

Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose so that these regions are free to bind IgG.

Converting From Linear Fow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

查看所有结果

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门