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描述
Ion Exchanger Type (value)
包裝
pack of 5 × 1 mL
製造商／商標名
Cytiva 11-0032-73
參數
flow rate
<4 ml/min flow rate
42 psi
床尺寸
7 mm × 25 mm
床體積
1 mL
柱內徑
7 mm
基質
highly cross-linked agarose
樣品平均尺寸
75 μm (d50v)
cleaning
2-14
工作範圍
2-12
適合性
suitable for bioprocess medium
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一般說明
HiTrap® Capto™ MMC is prepacked with Capto™ MMC, a multimodal BioProcess ion exchange medium. The multimodal functionality gives a different selectivity compared with traditional ion exchangers and it binds proteins at high or low ionic strength.
應用
The multimodal functionality gives a different selectivity compared with traditional ion exchangers including binding of Proteins regardless of ionic strength of the loading material . This means that the medium may be used for direct load of clarified feedstocks, without prior dilution to reduce the conductivity of the starting material. To reduce tedious sample preparation, the top filter in HiTrap® Capto™ MMC has been optimized for loading sonicated unclarified cell lysate directly on the column.
The Capto™ MMC ligand may interact with Proteins in many different ways. It contains a carboxyl group, and thus it is a weak cation exchanger. However, in addition to the ionic interactions several other types of interactions are involved, including hydrogen bonding and hydrophobic interaction.
The Capto™ MMC ligand may interact with Proteins in many different ways. It contains a carboxyl group, and thus it is a weak cation exchanger. However, in addition to the ionic interactions several other types of interactions are involved, including hydrogen bonding and hydrophobic interaction.
特點和優勢
- High dynamic binding capacity at high conductivity.
- Selectivity allows binding in the presence of salt.
- High volume throughput.
- HiTrap® Capto™ MMC can be used to bind Proteins at the conductivity of the feed material and to solve specific purification challenges.
儲存和穩定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
分析報告
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
其他說明
1)The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
法律資訊
Capto is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
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