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G8168

Sigma-Aldrich

G 418 二硫酸盐 溶液

50 mg/mL in H2O, 0.1 μm filtered, BioReagent, suitable for cell culture

别名:

抗生素G418

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About This Item

经验公式（希尔记法）:
C20H40N4O10 · 2H2SO4
CAS号:
108321-42-2
分子量:
692.71
MDL號碼:
MFCD00058314
分類程式碼代碼:
12352207
PubChem物質ID:
24895333
NACRES:
NA.76

生物源

Micromonospora echinospora

品質等級

200

無菌

0.1 μm filtered

產品線

BioReagent

形狀

solution

效力

50-60 mg per mL

分子量

692.7

包裝

pkg of 10 and 100 mL

濃度

50 mg/mL in H2O

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

顏色

colorless

溶解度

H2O: 50 mg/mL, clear

抗生素活性譜

parasites

作用方式

protein synthesis | interferes

儲存溫度

2-8°C

SMILES 字串

OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O

InChI

1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1

InChI 密鑰

UHEPSJJJMTWUCP-NKCAIAFTSA-N

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相关类别

一般說明

化学结构：氨基糖苷类

應用

G418二硫酸盐是一种氨基糖苷类抗生素，其结构与庆大霉素相似，用于选择和维持原核和真核细胞。不同细胞系具有不同的筛选和维持最佳浓度。 细菌和藻类最多需要5 μg/mL而动物细胞则可能需要300-500 μg/mL。

生化／生理作用

作用机制：G418可通过抑制70S和80S核糖体的合成来阻止多肽合成和蛋白质延伸。

抗菌谱：G418可选择经iNOS启动子构建体和新霉素抗性基因稳定转染的细胞，并显示出对原生动物和蠕虫的活性。

注意

该产品在2-8°C下可稳定储存两年。

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H317 - H334

危險分類

Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

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Kelvin Kai-Wan Hui et al.
Journal of cellular and molecular medicine, 23(3), 1784-1797 (2018-12-15)
Aberrant regulation of programmed cell death (PCD) has been tied to an array of human pathologies ranging from cancers to autoimmune disorders to diverse forms of neurodegeneration. Pharmacologic modulation of PCD signalling is therefore of central interest to a number
Joan Lin-Cereghino et al.
Yeast (Chichester, England), 25(4), 293-299 (2008-03-11)
The methylotrophic yeast, Pichia pastoris, is widely used as a host organism for the expression of heterologous proteins. Currently, the Zeocin and blasticidin resistance genes are the only dominant selectable markers that can be used for primary selection of transformants.
Jieru Li et al.
Nature structural & molecular biology, 27(11), 1032-1040 (2020-09-23)
Transcription activation by distal enhancers is essential for cell-fate specification and maintenance of cellular identities. How long-range gene regulation is physically achieved, especially within complex regulatory landscapes of non-binary enhancer-promoter configurations, remains elusive. Recent nanoscopy advances have quantitatively linked promoter
Luis M Molinos-Albert et al.
Retrovirology, 11, 44-44 (2014-06-10)
The MPER region of the HIV-1 envelope glycoprotein gp41 is targeted by broadly neutralizing antibodies. However, the localization of this epitope in a hydrophobic environment seems to hamper the elicitation of these antibodies in HIV infected individuals.We have quantified and
Lene Halkjær et al.
Frontiers in immunology, 11, 774-774 (2020-05-21)
We aimed at establishing a sensitive and robust assay for estimation of systemic complement activation at complement component C3 level in mouse and human plasma samples. In order to capture the activation products iC3b and C3dg in a specific and
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