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Merck

G7900

Sigma-Aldrich

Monoclonal Anti-Glycophorin A (α) antibody produced in mouse

ascites fluid, clone E4

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.41
共軛:
unconjugated
application:
Agglut.
FACS
IF
WB
無性繁殖:
E4, monoclonal
物種活性:
human
citations:
8
技術:
agglutination assay: 1:800 using human erythrocytes
flow cytometry: suitable using bone marrow nucleated cells
indirect immunofluorescence: suitable using bone marrow nucleated cells
western blot: suitable using extracts of human red blood cell ghosts

生物源

mouse

共軛

unconjugated

抗體表格

ascites fluid

抗體產品種類

primary antibodies

無性繁殖

E4, monoclonal

包含

15 mM sodium azide

物種活性

human

技術

agglutination assay: 1:800 using human erythrocytes
flow cytometry: suitable using bone marrow nucleated cells
indirect immunofluorescence: suitable using bone marrow nucleated cells
western blot: suitable using extracts of human red blood cell ghosts

同型

IgM

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... GYPA(2993)

特異性

By immunoblotting, the antibody localizes specifically the α, αδ, α2 bands in extracts of human red blood cell ghosts. The antibody (also cited as clone no. 15D4) binds to 8% of bone marrow nucleated cells in smears and tissue preparations using immunofluorescent microscopy or flow cytometry.

免疫原

human thymus

應用

Monoclonal Anti-Glycophorin A (α) antibody produced in mouse is suitable for:
  • agglutination assay at a working dilution of 1:800 using human erythrocytes
  • flow cytometry using bone marrow nucleated cells
  • indirect immunofluorescence using bone marrow nucleated cells
  • western blot using extracts of human red blood cell ghosts

生化/生理作用

Glycophorins (GP) are sialic acid-rich polypeptides (sialoglycoproteins) that are part of the erythrocyte membrane. They are denoted α, β, γ, δ based on the decreasing molar mass. GPA and GPB are the major constituents of the red cells. They may be present as single polypeptides (α and δ), as stable homodimers (α2 and δ2) and heterodimers (αδ). Depending upon the amino acid residues at positions 1 and 5, GPA carries blood group M or N. GPA is associated exclusively with erythroid cells. It is expressed in pronormoblasts and later erythroid cells. GPA has a cytoplasmic domain that interacts with cytoskeletal structure upon induction by binding to ligand. This interaction improves RBC membrane rigidity and reduces deformability.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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L L Jennings et al.
Neuropharmacology, 40(5), 722-731 (2001-04-20)
Nucleoside transport processes play an important role in human cells in salvage of nucleosides used in the biosynthesis of nucleic acids and in regulating endogenous adenosine concentrations in the human central nervous system (CNS). By altering the levels of adenosine
J A Chasis et al.
The Journal of clinical investigation, 75(6), 1919-1926 (1985-06-01)
Erythrocyte skeletal proteins are known to play an important role in determining membrane deformability. In order to see whether transmembrane proteins also influence deformability and, if so, whether this influence is mediated by an interaction with the membrane skeleton, we
D J Anstee
Vox sanguinis, 58(1), 1-20 (1990-01-01)
The surface of the human red blood cell is dominated by a small number of abundant blood group active proteins. The major proteins are the anion transport protein (band 3) which has AB(H) activity, and Glycophorin A which has MN
Monoclonal antibodies against glycophorins and other glycoproteins.
D J Anstee et al.
Journal of immunogenetics, 17(4-5), 301-308 (1990-08-01)
M J Telen et al.
Vox sanguinis, 52(3), 236-243 (1987-01-01)
Human erythrocyte membrane proteins express antigens which serve as markers for erythroid differentiation as well as targets for human blood group alloantibodies. We have produced and characterized a new panel of five monoclonal antibodies to erythrocyte membrane proteins. Three monoclonal

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